Rat IL-4 ELISA Kit | IL4 elisa kit

Rat IL-4 PicoKine ELISA Kit

Cat. Num. AAA175989

• Gene Names: Il4; Il4e12
• Reactivity: Rat
• Synonyms: IL-4; Rat IL-4 PicoKine ELISA Kit; Interleukin-4; interleukin 4; B cell growth factor 1; B cell stimulatory factor 1; BCGF 1; BCGF1; Binetrakin; BSF 1; BSF1; IL 4; Interleukin 4; Lymphocyte stimulatory factor 1; MGC79402; Pitrakinra; IL4 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat IL-4
• Sequence Length: 147

• Samples: Cell culture supernates and serum
• Assay Type: Sandwich
• Detection Range: 15.6pg/ml-1000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat IL-4 PicoKine ELISA Kit standard curve )

Related Product Information for IL4 elisa kit

Principle of the assay: rat IL-4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-4 has been precoated onto 96-well plates. Standards(E.coli,C25-S147) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat IL-4 amount of sample captured in plate.
Background: Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a proteinof 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functionalsimilarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 Rat IL-4 is a 13.5KDa glycoprotein, consisting of 147 amino acids in precursor form and 123amino acids in mature form.

NCBI and Uniprot Product Information

• NCBI GI #: 124339
• NCBI GeneID: 287287
• UniProt Accession #: P20096
• Molecular Weight: 16,247 Da
• NCBI Official Full Name: Interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: Il4
• NCBI Official Synonym Symbols: Il4e12
• NCBI Protein Information: interleukin-4; IL-4; BSF-1; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IGG differentiation factor
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: Il4
• UniProt Synonym Gene Names: Il-4; IL-4; BSF-1
• UniProt Entry Name: IL4_RAT

NCBI Description

Th2-type cytokine; may be involved in inflammatory response in eosinophils [RGD, Feb 2006]

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Cell cycle regulation; Secreted; Cytokine; Motility/polarity/chemotaxis

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: growth factor activity; cytokine activity; interleukin-4 receptor binding

Biological Process: negative regulation of osteoclast differentiation; positive regulation of isotype switching to IgG isotypes; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; female pregnancy; positive regulation of activated T cell proliferation; response to organic cyclic substance; positive regulation of interleukin-10 production; positive regulation of isotype switching to IgE isotypes; B cell costimulation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; response to nutrient; response to drug; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; positive regulation of mast cell degranulation; defense response to protozoan; positive regulation of immunoglobulin production; T-helper 1 cell lineage commitment; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of chemokine biosynthetic process; response to ethanol; innate immune response in mucosa; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of T cell differentiation; retina development in camera-type eye; response to cytokine stimulus; positive regulation of B cell activation; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; T-helper 2 cell differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of defense response to virus by host; negative regulation of transcription, DNA-dependent; negative regulation of T cell activation; regulation of T cell activation