Interleukin-33 Recombinant Protein | IL-33 recombinant protein

Human Interleukin-33 Recombinant

Cat. Num. AAA553201

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-33; Human Interleukin-33 Recombinant; IL1F11; NFHEV; interleukin-33; il33; il-33; IL-33 recombinant protein

• Host: E Coli
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-33 was lyophilized from 0.2 um filtered 1mg/ml in PBS buffer.
• Sequence: RRETTKRPSL KTGRKHKRHL VLAACQQQST VECFAFGISG VQKYTRALHD SSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI KVDSSENLCT ENILFKLSET
• Sequence Length: 228

• Domain: Actin Binding
• Host Note: Optimized DNA sequence encoding Human Interleukin-33 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin-33, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 18kDa.; ; Recombinant Interleukin-33 is a disulfide-linked monomer protein consisting of 160 amino acid residue subunits, and migrates as an approximately 18kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-33 recombinant protein

IL-33, a member of the IL-1 family, signals via a heterodimeric receptor complex consisting of ST2 and IL-1R accessory protein and triggers the activation of NF-B and all three MAPKs: p38, ERK1/2, and JNK1/2 in mast cells.IL-33 is expressed in multiple tissues and by several cell types such as dermal fibroblasts and small airway epithelial and bronchial smooth muscle cells. IL-33 activated IL-1-like signaling responses in mast cells and enhanced IL-5 and IL-13 production from murine Th2-polarized splenocytes.Moreover, it was found that both human and murine mast cells when stimulated in vitro with IL- 33 produced a wide spectrum of cytokines and chemokines. In addition, IL-33 enhanced IL-4-driven Th2 cell responses and acted as a selective chemoattractant for Th2 cell recruitment.

Product Categories/Family for IL-33 recombinant protein

Cytokines

References

• IL-33 induces il-13–dependent cutaneous fibrosis .J. Immunol., Feb 2010; 184: 1526 - 1535.
• serum levels of IL-33 and soluble st2 and their association with disease activity in systemic lupus erythematosus .Rheumatology, Dec 2009; 10.1093/rheumatology/kep402.
• caspase-1, caspase-8, and calpain are dispensable for IL-33 release by macrophages .J. Immunol., Dec 2009; 183: 7890 - 7897.
• IL-33 amplifies the polarization of alternatively activated macrophages that contribute to airway inflammation .J. Immunol., Nov 2009; 183: 6469 - 6477.
• IL-33 shifts macrophage polarization, promoting resistance against pseudomonas aeruginosa keratitis .Invest. Ophthalmol. Vis. Sci., Nov 2009; 10.1167/iovs.09-3983.