Human interleukin 33 ELISA Kit | IL33 elisa kit

Human Interleukin 33, IL-33 ELISA Kit

Cat. Num. AAA704373

• Gene Names: IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
• Reactivity: Human
• Synonyms: interleukin 33; Human Interleukin 33; IL-33 ELISA Kit; Human Interleukin 33 (IL-33) ELISA Kit; C9orf26; DKFZp586H0523; DVS27; NF-HEV; NFEHEV; RP11-575C20.2; DVS27-related protein; nuclear factor from high endothelial venules; IL33 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human IL-33. No significant cross-reactivity or interference between human IL-33 and analogues was observed.
• Sequence Length: 228

• Samples: Serum, plasma, urine
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL33 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-33 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-33 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-33 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-33 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 313851030
• NCBI GeneID: 90865
• NCBI Accession #: NP_001186569.1
• NCBI GenBank Nucleotide #: NM_001199640.1
• UniProt Accession #: O95760; Q2YEJ5; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4
• Molecular Weight: 30,759 Da
• NCBI Official Full Name: interleukin-33 isoform 2
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: IL33
• NCBI Official Synonym Symbols: DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26
• NCBI Protein Information: interleukin-33; IL-33; IL-1F11; DVS27-related protein; interleukin-1 family member 11; nuclear factor for high endothelial venules; nuclear factor from high endothelial venules
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: IL33
• UniProt Synonym Gene Names: C9orf26; IL1F11; NFHEV; IL-33; IL-1F11; NF-HEV
• UniProt Entry Name: IL33_HUMAN

NCBI Description

IL33 (MIM 608678) is a member of the IL1 (see MIM 147760) family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL4; MIM 147780). IL33 is a ligand for IL33R (IL1RL1; MIM 601203), an IL1 family receptor that is selectively expressed on Th2 cells and mast cells (summary by Yagami et al., 2010 [PubMed 20926795]).[supplied by OMIM, Jan 2011]

Uniprot Description

Function: Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (1 PublicationRef.3