Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody (C-term). MBS9201888 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Rabbit UBE2D4 Polyclonal Antibody | anti-UBE2D4 antibody
UBE2D4 Antibody (C-term)
Gene Names
UBE2D4; HBUCE1
Reactivity
Human (Predicted Reactivity: Xenopus)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
UBE2D4; Polyclonal Antibody; UBE2D4 Antibody (C-term); Ubiquitin-conjugating enzyme E2 D4; HBUCE1; Ubiquitin carrier protein D4; Ubiquitin-protein ligase D4; UBCH5D; anti-UBE2D4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This UBE2D4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-140 amino acids from the C-terminal region of human UBE2D4.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
111-140
Sequence Length
147
Applicable Applications for anti-UBE2D4 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody (C-term). MBS9201888 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from Jurkat cell line, using UBE2D4 Antibody (C-term). MBS9201888 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-UBE2D4 antibody
UBE2D4 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Product Categories/Family for anti-UBE2D4 antibody
References
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16649
NCBI Official Full Name
ubiquitin-conjugating enzyme E2 D4
NCBI Official Synonym Full Names
ubiquitin-conjugating enzyme E2D 4 (putative)
NCBI Official Symbol
UBE2D4
NCBI Official Synonym Symbols
HBUCE1
NCBI Protein Information
ubiquitin-conjugating enzyme E2 D4
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 D4
Protein Family
UniProt Gene Name
UBE2D4
UniProt Synonym Gene Names
UBCH5D
UniProt Entry Name
UB2D4_HUMAN
Uniprot Description
UBE2D4: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination. Belongs to the ubiquitin-conjugating enzyme family.
Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase
Chromosomal Location of Human Ortholog: 7p13
Molecular Function: protein binding; ubiquitin-protein ligase activity; ATP binding; ligase activity
Biological Process: protein ubiquitination
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Product Notes
The UBE2D4 ube2d4 (Catalog #AAA9201888) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 111-140. The UBE2D4 Antibody (C-term) reacts with Human (Predicted Reactivity: Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's UBE2D4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the UBE2D4 ube2d4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "UBE2D4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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