Human Interleukin-31 receptor subunit alpha ELISA Kit | IL31RA elisa kit

Human Interleukin-31 receptor subunit alpha ELISA Kit

Cat. Num. AAA764872

• Gene Names: Il31ra; GPL; CRL3; Glmr; NR10; GLM-R
• Reactivity: Human
• Synonyms: Interleukin-31 receptor subunit alpha; Human Interleukin-31 receptor subunit alpha ELISA Kit; IL31RA/CRL3/GLM-R/class I cytokine receptor/Cytokine receptor-like 3/GLMR/GP130 like receptor/Gp130-like monocyte receptor/hGLM-R/IL-31 receptor subunit alpha/IL-31R subunit alpha/IL-31R-alpha/interleukin 31 receptor A/soluble type I cytokine receptor CRL3; IL31RA elisa kit

• Reactivity: Human
• Sequence Length: 716

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for IL31RA elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL31RA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL31RA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL31RA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL31RA can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 225007641
• NCBI GeneID: 218624
• NCBI Accession #: NP_647460.2
• NCBI GenBank Nucleotide #: NM_139299.2
• Molecular Weight: 25,133 Da
• NCBI Official Full Name: interleukin-31 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 31 receptor A
• NCBI Official Symbol: Il31ra
• NCBI Official Synonym Symbols: GPL; CRL3; Glmr; NR10; GLM-R
• NCBI Protein Information: interleukin-31 receptor subunit alpha
• UniProt Protein Name: Interleukin-31 receptor subunit alpha
• Protein Family: Interleukin-31 receptor
• UniProt Gene Name: Il31ra
• UniProt Synonym Gene Names: Glmr; IL-31 receptor subunit alpha; IL-31R subunit alpha; IL-31R-alpha; IL-31RA; mGLM-R; Gp130-like receptor; NR10
• UniProt Entry Name: IL31R_MOUSE

Uniprot Description

IL31RA: Associates with OSMR to form the interleukin-31 receptor which activates STAT3 and to a lower extent STAT1 and STAT5. May function in skin immunity. Defects in IL31RA are the cause of amyloidosis primary localized cutaneous type 2 (PLCA2). PLCA2 is primary amyloidosis characterized by localized cutaneous amyloid deposition. This condition usually presents with itching (especially on the lower legs) and visible changes of skin hyperpigmentation and thickening that may be exacerbated by chronic scratching and rubbing. Primary localized cutaneous amyloidosis is often divided into macular and lichen subtypes although many affected individuals often show both variants coexisting. Lichen amyloidosis characteristically presents as a pruritic eruption of grouped hyperkeratotic papules with a predilection for the shins, calves, ankles and dorsa of feet and thighs. Papules may coalesce to form hyperkeratotic plaques that can resemble lichen planus, lichen simplex or nodular prurigo. Macular amyloidosis is characterized by small pigmented macules that may merge to produce macular hyperpigmentation, sometimes with a reticulate or rippled pattern. In macular and lichen amyloidosis, amyloid is deposited in the papillary dermis in association with grouped colloid bodies, thought to represent degenerate basal keratinocytes. The amyloid deposits probably reflect a combination of degenerate keratin filaments, serum amyloid P component, and deposition of immunoglobulins. Belongs to the type I cytokine receptor family. Type 2 subfamily. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: external side of plasma membrane; integral to membrane; membrane; plasma membrane

Molecular Function: cytokine binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity

Biological Process: acute inflammatory response to antigenic stimulus; glandular epithelial cell differentiation; immune system process; positive regulation of cell proliferation