Typical Testing Data/Standard Curve (for reference only)
Human hemoglobin, beta ELISA Kit | HBB elisa kit
Human beta-subunits of hemoglobin, HB-beta ELISA Kit
Gene Names
HBB; CD113t-C; beta-globin
Reactivity
Human
Synonyms
hemoglobin; beta; Human beta-subunits of hemoglobin; HB-beta ELISA Kit; CD113t-C; beta-globin; beta globin; beta globin chain; hemoglobin beta chain; HBB elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human HB-beta. No significant cross-reactivity or interference between human HB-beta and analogues was observed.
Sequence Length
147
Samples
Serum, plasma, lysate for RBC.
Assay Type
Competitive
Detection Range
0.12 ng/ml-500 ng/ml.
Sensitivity
0.12 ng/ml.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HBB elisa kit
Principle of the Assay: This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for HB-beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated HB-beta. A competitive inhibition reaction is launched between HB-beta (Standards or samples) and biotin-conjugated HB-beta with the pre-coated HB-beta antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of HB-beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,998 Da
NCBI Official Full Name
hemoglobin subunit beta
NCBI Official Synonym Full Names
hemoglobin, beta
NCBI Official Symbol
HBB
NCBI Official Synonym Symbols
CD113t-C; beta-globin
NCBI Protein Information
hemoglobin subunit beta; beta globin chain; hemoglobin beta chain
UniProt Protein Name
Hemoglobin subunit beta
Protein Family
UniProt Gene Name
HBB
UniProt Entry Name
HBB_HUMAN
NCBI Description
The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Involved in oxygen transport from the lung to the various peripheral tissues.2 PublicationsManual assertion based on experiment in:Ref.37
Research Articles on HBB
1. Beta-globin codon 17 (A --> T) is the most prevalent beta-thalassemia mutation in Guizhou Province.
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Product Notes
The Human HBB hbb (Catalog #AAA701530) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA701530 ELISA Kit recognizes Human HBB. It is sometimes possible for the material contained within the vial of "hemoglobin, beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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