Mouse IL-23 ELISA Kit | IL-23 elisa kit

Mouse IL-23 (Interleukin 23) ELISA Kit

Cat. Num. AAA2501597

• Gene Names: IL23A; P19; SGRF; IL-23; IL-23A; IL23P19
• Reactivity: Mouse
• Synonyms: IL-23; Mouse IL-23 (Interleukin 23) ELISA Kit; IL-23 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse IL-23. No significant cross-reactivity or interference between Mouse IL-23 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse IL-23 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 189

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-23 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-23 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-23. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-23 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-23, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse IL-23. You can calculate the concentration of Mouse IL-23 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7706702
• NCBI GeneID: 51561
• NCBI Accession #: NP_057668.1
• NCBI GenBank Nucleotide #: NM_016584.2
• UniProt Accession #: Q9NPF7; Q6NZ80; Q6NZ82; Q9H2A5
• Molecular Weight: 20,730 Da
• NCBI Official Full Name: interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23, alpha subunit p19
• NCBI Official Symbol: IL23A
• NCBI Official Synonym Symbols: P19; SGRF; IL-23; IL-23A; IL23P19
• NCBI Protein Information: interleukin-23 subunit alpha; IL-23 subunit alpha; IL-23-A; IL-23p19; JKA3 induced upon T-cell activation; interleukin 23 p19 subunit; interleukin-23 subunit p19; interleukin-six, G-CSF related factor
• UniProt Protein Name: Interleukin-23 subunit alpha
• UniProt Gene Name: IL23A
• UniProt Synonym Gene Names: SGRF; IL-23 subunit alpha; IL-23-A; IL-23p19
• UniProt Entry Name: IL23A_HUMAN

NCBI Description

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. [provided by RefSeq, Jul 2008]

Uniprot Description

IL23A: Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak- Stat signaling cascade, stimulates memory rather than naive T- cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 12q13.3

Molecular Function: protein binding; interleukin-23 receptor binding; cytokine activity

Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of T-helper 1 type immune response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; positive regulation of osteoclast differentiation; positive regulation of T cell mediated cytotoxicity; tissue remodeling; positive regulation of NK T cell proliferation; positive regulation of tyrosine phosphorylation of Stat4 protein; positive regulation of NF-kappaB import into nucleus; defense response to Gram-negative bacterium; positive regulation of activated T cell proliferation; positive regulation of interleukin-10 production; positive regulation of natural killer cell activation; positive regulation of tyrosine phosphorylation of Stat3 protein; T cell proliferation; positive regulation of T cell proliferation; inflammatory response; defense response to virus; positive regulation of interleukin-17 production; positive regulation of memory T cell differentiation; positive regulation of NK T cell activation; positive regulation of natural killer cell proliferation; positive regulation of tumor necrosis factor production; regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of tissue remodeling; positive regulation of interferon-gamma production; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of defense response to virus by host; positive regulation of inflammatory response