Interleukin-22 Recombinant Protein | IL-22 recombinant protein
Human Interleukin-22 Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
Interleukin-22; Human Interleukin-22 Recombinant; Human IL-22 Recombinant; ILTIF; ZCYTO18; il22; interleukin-22; il-22; IL-22 recombinant protein
Host
E Coli
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Interleukin-22 was lyophilized from 0.2um filtered concentrated (1.0mg/ml) solution in 500mM NaCl, 20mM Tris, pH 8.0.
Sequence
KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACI
Sequence Length
179
Domain
4 Helix Cytokine
Host Note
Optimized DNA sequence encoding Human Interleukin-22 mature chain was expressed in Escherichia Coli.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Molecular Weight Note
Native human IL-22 has a calculated molecular mass of approximately 17 kDa.
Recombinant IL-22 is a disulfide-linked homodimer protein consisting of 164 amino acid residue subunits, and migrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE.
Recombinant IL-22 is a disulfide-linked homodimer protein consisting of 164 amino acid residue subunits, and migrates as an approximately 17 kDa protein under reducing conditions in SDS-PAGE.
Preparation and Storage
Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
Avoid repeated freeze/thaw cycles.
Product Categories/Family for IL-22 recombinant protein
References
• deficiency of IL-22 contributes to a chronic inflammatory disease: pathogenetic mechanisms in acne inversa .J. Immunol., Jan 2011; 186: 1228 - 1239.
• functional characterization of a nonmammalian il-21: rainbow trout oncorhynchus mykiss il-21 upregulates the expression of the th cell signature cytokines ifn-, il-10, and IL-22 .J. Immunol., Jan 2011; 186: 708 - 721.
• IL-22+ cd4+ t cells are associated with therapeutic trichuris trichiura infection in an ulcerative colitis patient .Science Translational Medicine, Dec 2010; 2: 60ra88.
• stat3-dependent effects of IL-22 in human keratinocytes are counterregulated by sirtuin 1 through a direct inhibition of stat3 acetylation .FASEB J, Nov 2010; 10.1096/fj.10-172288.
• IL-22 induces an acute-phase response .J. Immunol., Nov 2010; 185: 5531 - 5538.
• functional characterization of a nonmammalian il-21: rainbow trout oncorhynchus mykiss il-21 upregulates the expression of the th cell signature cytokines ifn-, il-10, and IL-22 .J. Immunol., Jan 2011; 186: 708 - 721.
• IL-22+ cd4+ t cells are associated with therapeutic trichuris trichiura infection in an ulcerative colitis patient .Science Translational Medicine, Dec 2010; 2: 60ra88.
• stat3-dependent effects of IL-22 in human keratinocytes are counterregulated by sirtuin 1 through a direct inhibition of stat3 acetylation .FASEB J, Nov 2010; 10.1096/fj.10-172288.
• IL-22 induces an acute-phase response .J. Immunol., Nov 2010; 185: 5531 - 5538.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,011 Da
NCBI Official Full Name
interleukin-22
UniProt Protein Name
Interleukin-22
Protein Family
UniProt Gene Name
IL22
UniProt Synonym Gene Names
ILTIF; ZCYTO18; IL-22; IL-TIF
UniProt Entry Name
IL22_HUMAN
Uniprot Description
IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family.
Protein type: Secreted, signal peptide; Cytokine; Secreted
Chromosomal Location of Human Ortholog: 12q15
Cellular Component: extracellular space; extracellular region
Molecular Function: interleukin-22 receptor binding; cytokine activity
Biological Process: cell-cell signaling; response to glucocorticoid stimulus; acute-phase response; inflammatory response
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Product Notes
The IL-22 il22 (Catalog #AAA553228) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: KEASLADNNT DVRLIGEKLF HGVSMSERCY LMKQVLNFTL EEVLFPQSDR FQPYMQEVVP FLARLSNRLS TCHIEGDDLH IQRNVQKLKD TVKKLGESGE IKAIGELDLL FMSLRNACI. It is sometimes possible for the material contained within the vial of "Interleukin-22, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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