Rabbit CD57 Polyclonal Antibody | anti-CD57 antibody

CD57 (V35) Polyclonal Antibody

Cat. Num. AAA3003876

• Gene Names: B3GAT1; NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CD57; Polyclonal Antibody; CD57 (V35) Polyclonal Antibody; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; Beta-1; 3-glucuronyltransferase 1; Glucuronosyltransferase P; GlcAT-P; UDP-GlcUA: glycoprotein beta-1; 3-glucuronyltransferase; GlcUAT-P; B3GAT1; GLCATP; anti-CD57 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CD57 (V35) polyclonal antibody detects endogenous levels of CD57 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 334

• Applicable Applications for anti-CD57 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 3-50 of Human CD57.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of CD57 (V35) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-CD57 antibody

T lymphocytes displaying the natural killer (NK) cell marker CD57 (also designated Leu7) on their cell surface are distinguishable from other T cell subsets by their granular lymphocyte morphology and their clonal expansion in patients with AIDS and in recipients of bone marrow transplantation. CD57-positive cells have also been shown to localize to sites of certain tumors and large numbers of these cells are detected in the synovial fluid from patients suffering from rheumatoid arthritis.

NCBI and Uniprot Product Information

• NCBI GI #: 77695914
• NCBI GeneID: 27087
• NCBI Accession #: NP_061114.2
• NCBI GenBank Nucleotide #: NP_061114.2
• UniProt Accession #: Q9P2W7
• Molecular Weight: ~ 38kDa
• NCBI Official Full Name: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: beta-1,3-glucuronyltransferase 1
• NCBI Official Symbol: B3GAT1
• NCBI Official Synonym Symbols: NK1; CD57; HNK1; LEU7; NK-1; GLCATP; GLCUATP
• NCBI Protein Information: galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• UniProt Protein Name: Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• UniProt Gene Name: B3GAT1
• UniProt Synonym Gene Names: GlcUAT-P

NCBI Description

The protein encoded by this gene is a member of the glucuronyltransferase gene family. These enzymes exhibit strict acceptor specificity, recognizing nonreducing terminal sugars and their anomeric linkages. This gene product functions as the key enzyme in a glucuronyl transfer reaction during the biosynthesis of the carbohydrate epitope HNK-1 (human natural killer-1, also known as CD57 and LEU7). Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.