Interleukin-2 Recombinant Protein | IL-2 recombinant protein

Human Interleukin-2 Recombinant

Cat. Num. AAA553116

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Interleukin-2; Human Interleukin-2 Recombinant; T-cell growth factor; tcgf; aldesleukin; il2; il-2; IL-2 recombinant protein

• Host: E Coli
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant Interleukin-2 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in PBS, pH 7.2.
• Sequence: MYRMQLLSCI ALSLALVTNS APTSSSTKKT QLQLEHLLLD LQMILNGINN YKNPKLTRML TFKFYMPKKA TELKHLQCLE EELKPLEEVL NLAQSKNFHL RPRDLISNIN VIVLELKGSE TTFMCEYADE TATIVEFLNR WITFAQSIIS TLT
• Sequence Length: 153

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human Interleukin-2 mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Interleukin-2, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 15 kDa. Recombinant Interleukin-2 is a disulfide-linked monomer protein consisting of 133 amino acid residue subunits, migrates as an approximately 15 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for IL-2 recombinant protein

IL2 is produced mainly by T- cells expressing the surface antigen CD4 following cell activation by mitogens or allogen. Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T- cell proliferation and T- helper 1 (Th1) and Th2 effector T-cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the optimal survival and function of memory cells. In a regulatory role, IL-2 is important for the development, survival, and function of regulatory T cells, it enhances Fas- mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Thus, in its dual and contrasting functions, IL-2 contributes to both the induction and the termination of inflammatory immune responses.

Product Categories/Family for IL-2 recombinant protein

Cytokines

References

• vaccination with live leishmania major and cpg dna promotes IL-2 production by dermal dendritic cells and nk cell activation .Clin. Vaccine Immunol., Sep 2009; 10.1128/CVI.00249-09.
• erk and not p38 pathway is required for il-12 restoration of t cell IL-2 and ifn- in a rodent model of alcohol intoxication and burn injury .J. Immunol., Sep 2009; 183: 3955 - 3962.
• a glucocorticoid amplifies IL-2-induced selective expansion of cd4+cd25+foxp3+ regulatory t cells in vivo and suppresses graft-versus-host disease after allogeneic lymphocyte transplantation .Acta Biochim Biophys Sin, Sep 2009; 41: 781 - 791.
• self-peptides prolong survival in murine autoimmunity via reduced IL-2/il-7-mediated stat5 signaling, cd8 coreceptor, and v2 down-regulation .J. Immunol., Sep 2009; 183: 3130 - 3138.
• single nucleotide polymorphisms in genes for IL-2, il-6 and tnf- influence on osteolysis and survival of total hip arthroplasty .J Bone Joint Surg Br Proceedings, Sep 2009; 91-B: 473.