Interleukin 1 Alpha (IL1a) Recombinant Protein | IL1a recombinant protein

Recombinant Interleukin 1 Alpha (IL1a)

Cat. Num. AAA2009970

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 1 Alpha (IL1a); Recombinant Interleukin 1 Alpha (IL1a); IL1a recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- SVPYSFQSNL KFKYQRSIKK GAVITDAMHQ SLIRESNGQH LKAMHVVDRK HEVKFDIDGY VSTATRIRPV TLKISKTQLY VCAQEEGQPV LLKE
• Sequence Length: 204

• Applicable Applications for IL1a recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Cavia (Guinea pig)
• Expression System: Prokaryotic expression
• Residues: Ser111~Glu204 (Accession # Q60480) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 2497333
• UniProt Accession #: Q60480
• Molecular Weight: 16.5kDa
• NCBI Official Full Name: Interleukin-1 alpha
• UniProt Protein Name: Interleukin-1 alpha
• Protein Family: Interleukin
• UniProt Gene Name: IL1A
• UniProt Synonym Gene Names: IL-1 alpha
• UniProt Entry Name: IL1A_CAVPO

Uniprot Description

Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells

By similarity.

Subunit structure: Monomer

By similarity.

Subcellular location: Secreted. Note: The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Domain: The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.

Sequence similarities: Belongs to the IL-1 family.