Interleukin 2 Receptor Alpha (IL2Ra) Recombinant Protein | IL2Ra recombinant protein

Recombinant Interleukin 2 Receptor Alpha (IL2Ra)

Cat. Num. AAA2018733

• Gene Names: Il2ra; CD25; Il2r; Ly-43
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Interleukin 2 Receptor Alpha (IL2Ra); Recombinant Interleukin 2 Receptor Alpha (IL2Ra); IL2Ra recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- ELCLYDPPE VPNATFKALS YKNGTILNCE CKRGFRRLKE LVYMRCLGNS WSSNCQCTSN SHDKSRKQVT AQLEHQKEQQ TTTDMQKPTQ SMHQENLTGH CREPPPWKHE DSKRIYHFVE GQSVHYECIP GYKALQRGPA ISICKMKCGK TGWTQPQLTC VDEREHHRFL ASEESQGSRN SSPESETSCP ITTTDFPQPT ETTAMTETFV LTMEY
• Sequence Length: 268

• Applicable Applications for IL2Ra recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Glu22~Tyr235 (Accession # P01590) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 254540187
• NCBI GeneID: 16184
• NCBI Accession #: NP_032393.3
• NCBI GenBank Nucleotide #: NM_008367.3
• UniProt Accession #: P01590; Q61731
• Molecular Weight: 30.3kDa
• NCBI Official Full Name: interleukin-2 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 2 receptor, alpha chain
• NCBI Official Symbol: Il2ra
• NCBI Official Synonym Symbols: CD25; Il2r; Ly-43
• NCBI Protein Information: interleukin-2 receptor subunit alpha; IL2-RA; IL-2-RA; p55 chain; IL-2R alpha chain; IL-2R subunit alpha; IL-2 receptor subunit alpha
• UniProt Protein Name: Interleukin-2 receptor subunit alpha
• Protein Family: Interleukin-2 receptor
• UniProt Gene Name: Il2ra
• UniProt Synonym Gene Names: Il2r; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
• UniProt Entry Name: IL2RA_MOUSE

Uniprot Description

IL2RA: Receptor for interleukin-2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit.

Protein type: Membrane protein, integral

Cellular Component: cell surface; membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; interleukin-2 receptor activity; drug binding; interleukin-2 binding

Biological Process: Notch signaling pathway; regulation of T cell homeostatic proliferation; negative regulation of immune response; negative regulation of defense response to virus; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; negative regulation of T cell proliferation; inflammatory response to antigenic stimulus; positive regulation of T cell differentiation; negative regulation of inflammatory response; T cell homeostasis; positive regulation of T cell proliferation; immune response; activated T cell apoptosis