Rabbit JAG1 / Jagged 1 Polyclonal Antibody | anti-JAG1 antibody

Rabbit Polyclonal (IgG) to Human JAG1 / Jagged 1

Cat. Num. AAA242046

• Gene Names: JAG1; AGS; AHD; AWS; HJ1; CD339; JAGL1
• Reactivity: Human; Predicted Reactivity: Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: JAG1 / Jagged 1; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human JAG1 / Jagged 1; Anti-JAG1 / Jagged 1 Antibody (aa110-125) IHC-plus; JAG1; AGS; AHD; Alagille syndrome; CD339 antigen; HJ1; Jagged1; JAGL1; Jagged; Jagged 1; Protein jagged-1; AWS; CD339; Soluble protein jagged; Human JAG1; anti-JAG1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Jagged1 (JAG1).
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1218

• Applicable Applications for anti-JAG1 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:2500 - 1:10000), ICC, IF (1:100 - 1:500), IHC-P (5 ug/ml), WB (1:250 - 1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide corresponding to aa 110-125 of human Jagged-1protein.
• Immunogen Type: Synthetic peptide
• Immunogen: JAG1 / Jagged 1 antibody was raised against synthetic peptide corresponding to aa 110-125 of human Jagged-1protein.
• Antigen Modification: aa110-125
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-JAG1 / Jagged 1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-JAG1 antibody

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophila jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophila jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis.

NCBI and Uniprot Product Information

• NCBI GI #: 4557679
• NCBI GeneID: 182
• NCBI Accession #: NP_000205.1
• NCBI GenBank Nucleotide #: NM_000214.2
• UniProt Accession #: P78504; O14902; O15122; Q15816; A0AV43; B4DYR1; E9PCF9
• Molecular Weight: 116,579 Da
• NCBI Official Full Name: protein jagged-1
• NCBI Official Synonym Full Names: jagged 1
• NCBI Official Symbol: JAG1
• NCBI Official Synonym Symbols: AGS; AHD; AWS; HJ1; CD339; JAGL1
• NCBI Protein Information: protein jagged-1
• UniProt Protein Name: Protein jagged-1
• Protein Family: Protein jagged
• UniProt Gene Name: JAG1
• UniProt Synonym Gene Names: JAGL1; Jagged1; hJ1
• UniProt Entry Name: JAG1_HUMAN

NCBI Description

The jagged 1 protein encoded by JAG1 is the human homolog of the Drosophilia jagged protein. Human jagged 1 is the ligand for the receptor notch 1, the latter a human homolog of the Drosophilia jagged receptor notch. Mutations that alter the jagged 1 protein cause Alagille syndrome. Jagged 1 signalling through notch 1 has also been shown to play a role in hematopoiesis. [provided by RefSeq, Jul 2008]

Uniprot Description

JAG1: Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation. Enhances fibroblast growth factor-induced angiogenesis (in vitro). Interacts with NOTCH2 and NOTCH3. Interacts with NOTCH1 (in the presence of calcium ions). Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p12.1-p11.23

Cellular Component: membrane; integral to plasma membrane; apical part of cell; extracellular region; plasma membrane

Molecular Function: protein binding; growth factor activity; calcium ion binding; Notch binding; structural molecule activity

Biological Process: nervous system development; endothelial cell differentiation; Notch signaling pathway; positive regulation of myeloid cell differentiation; T cell mediated immunity; Notch receptor processing; auditory receptor cell differentiation; cell fate determination; negative regulation of fat cell differentiation; regulation of cell migration; regulation of cell proliferation; myoblast differentiation; keratinocyte differentiation; positive regulation of osteoblast differentiation; negative regulation of neuron differentiation; response to muramyl dipeptide; morphogenesis of an epithelial sheet; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; blood vessel remodeling; angiogenesis; positive regulation of Notch signaling pathway

Disease: Tetralogy Of Fallot; Alagille Syndrome 1