Mouse IL-5 (Interleukin 5) CLIA Kit | IL-5 clia kit

Mouse IL-5 (Interleukin 5) CLIA Kit

Cat. Num. AAA2531664

• Gene Names: IL5; EDF; TRF; IL-5
• Reactivity: Mouse
• Synonyms: IL-5 (Interleukin 5); Mouse IL-5 (Interleukin 5) CLIA Kit; IL5; EDF; TRF; E-CSF; ESGF; IL-5 clia kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant IL-5. No significant cross-reactivity or interference between IL-5 and analogues was observed.
• Sequence Length: 134

• Assay Type: Sandwich
• Detection Range: 7.813-500pg/mL
• Sensitivity: Min: 4.688pg/mL; Max: 500pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-5 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of IL-5 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to IL-5. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of IL-5. You can calculate the concentration of IL-5 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.2
• Molecular Weight: 15,238 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5
• UniProt Protein Name: Interleukin-5
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region; intracellular

Molecular Function: interleukin-5 receptor binding; protein binding; growth factor activity; cytokine activity

Biological Process: epidermal growth factor receptor signaling pathway; axon guidance; fibroblast growth factor receptor signaling pathway; activation of MAPKK activity; nerve growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MAPKKK cascade; positive regulation of JAK-STAT cascade; positive regulation of immunoglobulin secretion; positive regulation of peptidyl-tyrosine phosphorylation; small GTPase mediated signal transduction; Ras protein signal transduction; positive regulation of B cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of transcription factor activity; vascular endothelial growth factor receptor signaling pathway; inflammatory response; positive regulation of eosinophil differentiation