Typical Testing Data/Standard Curve (for reference only)
Canine Rho Guanine Nucleotide Exchange Factor 16 ELISA Kit | ARHGEF16 elisa kit
Canine Rho Guanine Nucleotide Exchange Factor 16 ELISA Kit
Gene Names
ARHGEF16; NBR; GEF16
Reactivity
Canine
Synonyms
Rho Guanine Nucleotide Exchange Factor 16; Canine Rho Guanine Nucleotide Exchange Factor 16 ELISA Kit; ARHGEF16 elisa kit
Reactivity
Canine
Sequence Length
709
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
2ng/L-600ng/L
Sensitivity
1.79ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ARHGEF16 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Canine Prostaglandin FM (also known as PGFM) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine PGFM antibody. PGFM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine PGFM Antibody is added and binds to PGFM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PGFM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine PGFM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Canine PGFM antibody. PGFM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine PGFM Antibody is added and binds to PGFM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PGFM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine PGFM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
rho guanine nucleotide exchange factor 16
NCBI Official Synonym Full Names
Rho guanine nucleotide exchange factor 16
NCBI Official Symbol
ARHGEF16
NCBI Official Synonym Symbols
NBR; GEF16
NCBI Protein Information
rho guanine nucleotide exchange factor 16
UniProt Protein Name
Rho guanine nucleotide exchange factor 16
Protein Family
UniProt Gene Name
ARHGEF16
UniProt Synonym Gene Names
EPHEXIN4; NBR
UniProt Entry Name
ARHGG_HUMAN
NCBI Description
Although the specific function of this protein is not known yet, it is thought to be involved in protein-protein and protein-lipid interactions. [provided by RefSeq, Jul 2008]
Uniprot Description
ARHGEF16: a Rho guanine exchange factor protein that contains 1 DH, 1 PH and 1 SH3 domain.
Protein type: GEFs; Motility/polarity/chemotaxis; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 1p36.3
Cellular Component: cytosol
Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; Rho GTPase binding; receptor tyrosine kinase binding; PDZ domain binding
Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction
Research Articles on ARHGEF16
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Product Notes
The Canine ARHGEF16 arhgef16 (Catalog #AAA1603419) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1603419 ELISA Kit recognizes Canine ARHGEF16. It is sometimes possible for the material contained within the vial of "Rho Guanine Nucleotide Exchange Factor 16, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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