Typical Testing Data/Standard Curve (for reference only)
Monkey Soluble Interleukin 2beta receptor ELISA Kit | IL-2sRbeta elisa kit
Monkey Soluble Interleukin 2beta receptor ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: sIL1R4/ST2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-sIL1R4/ST2 antibody and an sIL1R4/ST2-HRP conjugate. The assay sample and buffer are incubated together with sIL1R4/ST2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sIL1R4/ST2 concentration since sIL1R4/ST2 from samples and sIL1R4/ST2-HRP conjugate compete for the anti-sIL1R4/ST2 antibody binding site. Since the number of sites is limited, as more sites are occupied by sIL1R4/ST2 from the sample, fewer sites are left to bind sIL1R4/ST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sIL1R4/ST2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein. [provided by RefSeq, Jul 2008]
Uniprot Description
IL2RB: Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Non-covalent dimer of an alpha and a beta subunit. IL2R exists in 3 different forms: a high affinity dimer, an intermediate affinity monomer (beta subunit), and a low affinity monomer (alpha subunit). The high and intermediate affinity forms also associate with a gamma subunit. Interacts with SHB upon interleukin stimulation. Interacts with HTLV-1 accessory protein p12I. Belongs to the type I cytokine receptor family. Type 4 subfamily.
Protein type: Receptor, cytokine; Membrane protein, integral
Chromosomal Location of Human Ortholog: 22q13.1
Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: interleukin-2 receptor activity; interleukin-2 binding
Biological Process: viral reproduction; cytokine and chemokine mediated signaling pathway; protein complex assembly; signal transduction; negative regulation of apoptosis
Research Articles on IL-2sRbeta
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Product Notes
The Monkey IL-2sRbeta il2rb (Catalog #AAA743259) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA743259 ELISA Kit recognizes Monkey IL-2sRbeta. It is sometimes possible for the material contained within the vial of "Soluble Interleukin 2beta receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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