Human Calcineurin subunit B type 1 ELISA Kit | PPP3R1 elisa kit

Human Calcineurin subunit B type 1 ELISA Kit

Cat. Num. AAA763039

• Gene Names: PPP3R1; CNB; CNB1; CALNB1
• Reactivity: Human
• Synonyms: Calcineurin subunit B type 1; Human Calcineurin subunit B type 1 ELISA Kit; PPP3R1/CALNB1/MCIP1/PP2B beta 1/CNA2/CNB1/Protein phosphatase 2B regulatory subunit 1/protein phosphatase 2B regulatory subunit B alpha/Protein phosphatase 3 regulatory subunit B alpha isoform 1/protein phosphatase 3; regulatory subunit B; alpha/type I (19kDa); PPP3R1 elisa kit

• Reactivity: Human
• Sequence Length: 170

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PPP3R1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PPP3R1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PPP3R1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PPP3R1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PPP3R1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506025
• NCBI GeneID: 5534
• NCBI Accession #: NP_000936.1
• NCBI GenBank Nucleotide #: NM_000945.3
• Molecular Weight: 19,300 Da
• NCBI Official Full Name: calcineurin subunit B type 1
• NCBI Official Synonym Full Names: protein phosphatase 3 regulatory subunit B, alpha
• NCBI Official Symbol: PPP3R1
• NCBI Official Synonym Symbols: CNB; CNB1; CALNB1
• NCBI Protein Information: calcineurin subunit B type 1
• UniProt Protein Name: Calcineurin subunit B type 1
• Protein Family: Calcineurin
• UniProt Gene Name: PPP3R1
• UniProt Synonym Gene Names: CNA2; CNB
• UniProt Entry Name: CANB1_HUMAN

Uniprot Description

PPP3R1: Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Belongs to the calcineurin regulatory subunit family.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Protein phosphatase, regulatory subunit; Apoptosis

Chromosomal Location of Human Ortholog: 2p15

Cellular Component: calcineurin complex; cytosol; mitochondrion; nucleoplasm; sarcolemma

Molecular Function: calcium ion binding; calcium-dependent protein serine/threonine phosphatase activity; calmodulin binding; protein binding; protein domain specific binding

Biological Process: apoptosis; calcineurin-NFAT signaling pathway; innate immune response; positive regulation of NFAT protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; programmed cell death; protein amino acid dephosphorylation; stimulatory C-type lectin receptor signaling pathway