Canine Interleukin 17A ELISA Kit | IL-17A elisa kit

Canine Interleukin 17A ELISA Kit

Cat. Num. AAA740514

• Gene Names: IL17A; IL17; CTLA8; IL-17; IL-17A
• Reactivity: Canine
• Synonyms: Interleukin 17A; Canine Interleukin 17A ELISA Kit; IL-17A elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This IL-17A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine IL-17A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-17A elisa kit

Principle of the assay: IL-17A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-17A antibody and an IL-17A-HRP conjugate. The assay sample and buffer are incubated together with IL-17A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-17A concentration since IL-17A from samples and IL-17A-HRP conjugate compete for the anti-IL-17A antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-17A from the sample, fewer sites are left to bind IL-17A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-17A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL-17A elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 4504651
• NCBI GeneID: 3605
• NCBI Accession #: NP_002181.1
• NCBI GenBank Nucleotide #: NM_002190.2
• Molecular Weight: 17,504 Da
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: IL17A
• NCBI Official Synonym Symbols: IL17; CTLA8; IL-17; IL-17A
• NCBI Protein Information: interleukin-17A; CTLA-8; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• UniProt Protein Name: Interleukin-17A
• UniProt Gene Name: IL17A
• UniProt Synonym Gene Names: CTLA8; IL17; IL-17; IL-17A; CTLA-8
• UniProt Entry Name: IL17_HUMAN

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; external side of plasma membrane

Molecular Function: cytokine activity

Biological Process: cell death; cell-cell signaling; apoptosis; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response