Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Interleukin 17A ELISA Kit | IL-17A elisa kit
Guinea pig Interleukin 17A ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-12RB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-12RB2 antibody and an IL-12RB2-HRP conjugate. The assay sample and buffer are incubated together with IL-12RB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-12RB2 concentration since IL-12RB2 from samples and IL-12RB2-HRP conjugate compete for the anti-IL-12RB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-12RB2 from the sample, fewer sites are left to bind IL-12RB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-12RB2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]
Uniprot Description
IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6p12
Cellular Component: extracellular space; external side of plasma membrane
Molecular Function: cytokine activity
Biological Process: cell death; cell-cell signaling; positive regulation of osteoclast differentiation; apoptosis; positive regulation of interleukin-23 production; protein amino acid glycosylation; positive regulation of transcription from RNA polymerase II promoter; immune response; inflammatory response
Research Articles on IL-17A
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Product Notes
The Guinea Pig IL-17A il17a (Catalog #AAA727428) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727428 ELISA Kit recognizes Guinea Pig IL-17A. It is sometimes possible for the material contained within the vial of "Interleukin 17A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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