Insulin Like Growth Factor Binding Protein 3 (IGFBP3) Recombinant Protein | IGFBP3 recombinant protein

Recombinant Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

Cat. Num. AAA2009715

• Gene Names: Igfbp3; IGF-BP3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Insulin Like Growth Factor Binding Protein 3 (IGFBP3); Recombinant Insulin Like Growth Factor Binding Protein 3 (IGFBP3); IGFBP3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-NTTE SEEDHNAGSV ESQVVPSTHR VTDSKFHPLH SKMEVIIKGQ ARDSQRYKVD YESQSTDTQN FSSESKRETE YGPCRREMED TLNHLKFLNV LSPRGVHIPN CDKKGFYKKK QCRPSKGRKR GFCWCVDKYG QPLP
• Sequence Length: 292

• Applicable Applications for IGFBP3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asn137~Pro274 (Accession # P15473) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 298231182
• NCBI GeneID: 24484
• NCBI Accession #: NP_036720.2
• NCBI GenBank Nucleotide #: NM_012588.2
• UniProt Accession #: P15473
• Molecular Weight: 22kDa
• NCBI Official Full Name: insulin-like growth factor-binding protein 3
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 3
• NCBI Official Symbol: Igfbp3
• NCBI Official Synonym Symbols: IGF-BP3
• NCBI Protein Information: insulin-like growth factor-binding protein 3; IBP-3; IGFBP-3; IGF-binding protein 3; insulin-like growth factor-binding protein (IGF-BP3)
• UniProt Protein Name: Insulin-like growth factor-binding protein 3
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: Igfbp3
• UniProt Synonym Gene Names: Igfbp-3; IBP-3; IGF-binding protein 3; IGFBP-3
• UniProt Entry Name: IBP3_RAT

NCBI Description

an insulin growth factor (Igf) binding protein; involved in modulating IGF-I action [RGD, Feb 2006]

Uniprot Description

IGFBP3: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R.

Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide

Cellular Component: extracellular space; nucleus

Molecular Function: insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; fibronectin binding; insulin-like growth factor II binding; protein tyrosine phosphatase activator activity

Biological Process: positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of catalytic activity; regulation of insulin-like growth factor receptor signaling pathway; negative regulation of smooth muscle cell proliferation; positive regulation of apoptosis; negative regulation of smooth muscle cell migration; protein amino acid phosphorylation; positive regulation of myoblast differentiation; osteoblast differentiation; negative regulation of cell proliferation; negative regulation of protein amino acid phosphorylation; positive regulation of MAPKKK cascade; regulation of growth; regulation of cell growth