Human IGFBP-1 ELISA Kit | IGFBP1 elisa kit

Human IGFBP-1 PicoKine ELISA Kit

Cat. Num. AAA175858

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Reactivity: Human
• Synonyms: IGFBP-1; Human IGFBP-1 PicoKine ELISA Kit; Insulin-like growth factor-binding protein 1; insulin-like growth factor binding protein 1; AFBP; hIGFBP-1; IBP1; IGF-binding protein 1; IGF-BP25; Placental protein 12; PP12; IGFBP1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human IGFBP-1
• Sequence Length: 259

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA) and urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human IGFBP-1 PicoKine ELISA Kit standard curve )

Related Product Information for IGFBP1 elisa kit

Principle of the assay: human IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO,A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-1 amount of sample captured in plate.
Background: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesizedby human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copygene, located on chromosome 7.5 The standard product used in this kit is recombinant human IGFBP-1, consistingof 234 amino acids with the molecular mass of 25KDa..

NCBI and Uniprot Product Information

• NCBI GI #: 124055
• NCBI GeneID: 3484
• UniProt Accession #: P08833; Q8IYP5; A4D2F4; D3DVL9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: Insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGFBP-1; binding protein-25; binding protein-26; binding protein-28; placental protein 12; IGF-binding protein 1; amniotic fluid binding protein; growth hormone independent-binding protein; alpha-pregnancy-associated endometrial globulin
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
• UniProt Entry Name: IBP1_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: extracellular space; extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; regulation of insulin-like growth factor receptor signaling pathway; tissue regeneration; unfolded protein response; insulin receptor signaling pathway; signal transduction; positive regulation of cell growth