Typical Testing Data/Standard Curve (for reference only)
Rat Myoglobin ELISA Kit | MYO/MB elisa kit
Rat Myoglobin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MYO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MYO antibody and an MYO-HRP conjugate. The assay sample and buffer are incubated together with MYO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MYO concentration since MYO from samples and MYO-HRP conjugate compete for the anti-MYO antibody binding site. Since the number of sites is limited, as more sites are occupied by MYO from the sample, fewer sites are left to bind MYO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MYO concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.
Protein type: Carrier
Chromosomal Location of Human Ortholog: 22q13.1
Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity
Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hypoxia; response to hormone stimulus; brown fat cell differentiation; enucleate erythrocyte differentiation
Research Articles on MYO/MB
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Product Notes
The Rat MYO/MB mb (Catalog #AAA720631) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720631 ELISA Kit recognizes Rat MYO/MB. It is sometimes possible for the material contained within the vial of "Myoglobin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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