Human IGFBP-1 ELISA Kit | IGFBP-1 elisa kit

IGFBP-1 Human ELISA Kit

Cat. Num. AAA355331

• Gene Names: IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• Reactivity: Human
• Applications: ELISA
• Synonyms: IGFBP-1; IGFBP-1 Human ELISA Kit; Human IGFBP-1; IGFBP-1 elisa kit

• Reactivity: Human

• Applicable Applications for IGFBP-1 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of IGFBP-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml-2000 pg/ml
• Sensitivity: < 1pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGFBP-1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGFBP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-1 can be calculated.

Background: Insulin-like growth factor-binding protein 1 (IGFBP-1) also known as placental protein 12 (PP12) is a member of the Insulin-like growth factor-binding protein (IGFBP) family. It is synthesized in liver, secretory endometrium, and decidua. The IGFBP1 gene has 4 exons, spans 5.9 kb, and mapped to 7p13-p12. This protein has an IGFBP domain and a type-I thyroglobulin domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Leu and George (2007) concluded that IGFBP1 is a negative regulator of the p53/BAK-dependent pathway of apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4504615
• NCBI GeneID: 3484
• NCBI Accession #: NP_000587.1
• NCBI GenBank Nucleotide #: NM_000596.2
• UniProt Accession #: P08833; Q8IYP5; A4D2F4; D3DVL9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein 1
• NCBI Official Symbol: IGFBP1
• NCBI Official Synonym Symbols: AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
• NCBI Protein Information: insulin-like growth factor-binding protein 1; IBP-1; IGFBP-1; binding protein-25; binding protein-26; binding protein-28; placental protein 12; IGF-binding protein 1; amniotic fluid binding protein; growth hormone independent-binding protein; alpha-pregnancy-associated endometrial globulin
• UniProt Protein Name: Insulin-like growth factor-binding protein 1
• UniProt Gene Name: IGFBP1
• UniProt Synonym Gene Names: IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12
• UniProt Entry Name: IBP1_HUMAN

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. Ref.20

Subunit structure: Binds equally well IGF1 and IGF2.

Subcellular location: Secreted.

Post-translational modification: Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. Ref.14 Ref.18 Ref.20

Sequence similarities: Contains 1 IGFBP N-terminal domain.Contains 1 thyroglobulin type-1 domain.

Sequence caution: The sequence AAA52540.1 differs from that shown. Reason: Frameshift at positions 55 and 71.