Mouse IGF1 ELISA Kit | IGF1 elisa kit

Mouse IGF1 ELISA Kit

Cat. Num. AAA825061

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: IGF1; Mouse IGF1 ELISA Kit; IBP1; Insulin-like growth factor I; IGF-I; Mechano growth factor; MGF; Somatomedin-C; IGF1 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse CD274 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse CD274 proteins within the range of 125 pg/ml-8000 pg/ml.
• Sequence Length: 153

• Applicable Applications for IGF1 elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: 60 pg/ml.
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IGF1 elisa kit

Principle of the Assay: The MBS Mouse CD274 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD274 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Mouse CD274 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD274 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse CD274 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD274 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm!!Background/Introduction: Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.

NCBI and Uniprot Product Information

• NCBI GI #: 163659894
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104745.1
• NCBI GenBank Nucleotide #: NM_001111275.1
• UniProt Accession #: P05017; P05018; Q6LDP4; Q8C4U6
• Molecular Weight: 17,623 Da
• NCBI Official Full Name: insulin-like growth factor I isoform 4 preproprotein
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I; somatomedin
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: Igf1
• UniProt Synonym Gene Names: Igf-1; IGF-I
• UniProt Entry Name: IGF1_MOUSE

Uniprot Description

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space; cell soma; extracellular region; intracellular

Molecular Function: integrin binding; protein serine/threonine kinase activator activity; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; steroid binding; insulin receptor binding

Biological Process: positive regulation of transcription, DNA-dependent; chondroitin sulfate proteoglycan biosynthetic process; regulation of protein metabolic process; exocrine pancreas development; water homeostasis; positive regulation of glucose import; positive regulation of fibroblast proliferation; proteoglycan biosynthetic process; inner ear development; positive regulation of DNA binding; muscle hypertrophy; nervous system development; positive regulation of mitosis; positive regulation of protein import into nucleus, translocation; regulation of establishment and/or maintenance of cell polarity; regulation of calcium ion transport; positive regulation of cell growth; positive regulation of phosphoinositide 3-kinase cascade; cell activation; positive regulation of peptidyl-tyrosine phosphorylation; insulin-like growth factor receptor signaling pathway; branching morphogenesis of a tube; response to heat; regulation of gene expression; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; alveolus development; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of smooth muscle cell proliferation; myoblast proliferation; positive regulation of glycogen biosynthetic process; negative regulation of caspase activity; positive regulation of smooth muscle cell migration; detection of mechanical stimulus involved in sensory perception; positive regulation of activated T cell proliferation; regulation of translation; negative regulation of cell proliferation; glial cell differentiation; positive regulation of MAPKKK cascade; mammary gland development; positive regulation of cell proliferation; regulation of steroid hormone receptor signaling pathway; positive regulation of granule cell precursor proliferation; regulation of protein amino acid phosphorylation; phosphoinositide-mediated signaling; multicellular organism growth; myotube cell development; satellite cell compartment self-renewal involved in skeletal muscle regeneration; memory; regulation of cell proliferation; myoblast differentiation; positive regulation of protein kinase B signaling cascade; cell proliferation; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of glycolysis; blood vessel remodeling; positive regulation of Ras protein signal transduction; cell development; positive regulation of DNA replication; lung development