Typical Testing Data/Standard Curve
Human IFN alpha 1 ELISA Kit | IFNA1 elisa kit
Human IFN alpha 1 ELISA Kit
Gene Names
IFNA1; IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
Reactivity
Human
Applications
ELISA
Synonyms
IFN alpha 1; Human IFN alpha 1 ELISA Kit; Interferon alpha-1/13; IFN-alpha-1/13; Interferon alpha-D; LeIF D; IFNA1 elisa kit
Reactivity
Human
Specificity
The Human IFN alpha 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IFN alpha 1 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
Sequence Length
189
Applicable Applications for IFNA1 elisa kit
Sandwich ELISA (EIA)
Samples
Cell Culture Supernatants, Serum, Plasma.
Sensitivity
7 pg/ml.
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Related Product Information for IFNA1 elisa kit
Principle of the Assay||The Human IFN alpha 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IFN alpha 1 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IFN alpha 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and IFN alpha 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IFN alpha 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IFN alpha 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection; they also affect cell differentiation, growth, surface antigen expression and immunoregulation. There are three naturally occurring interferons: alpha, beta and gamma. IFN-alpha is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. When binding to discrete cell surface receptors on target cells, IFN-alpha induces rapid changes in Jak/Stat phosphorylation, which intiates the Jak/Stat signaling pathway. IFN-alpha signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (beta and epsilon). All IFN-alpha signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
Background/Introduction: Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection; they also affect cell differentiation, growth, surface antigen expression and immunoregulation. There are three naturally occurring interferons: alpha, beta and gamma. IFN-alpha is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin. When binding to discrete cell surface receptors on target cells, IFN-alpha induces rapid changes in Jak/Stat phosphorylation, which intiates the Jak/Stat signaling pathway. IFN-alpha signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (beta and epsilon). All IFN-alpha signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,725 Da
NCBI Official Full Name
Interferon alpha-1/13
NCBI Official Synonym Full Names
interferon, alpha 1
NCBI Official Symbol
IFNA1
NCBI Official Synonym Symbols
IFL; IFN; IFNA@; IFNA13; IFN-ALPHA; IFN-alphaD
NCBI Protein Information
interferon alpha-1/13
UniProt Protein Name
Interferon alpha-1/13
Protein Family
UniProt Gene Name
IFNA1
UniProt Synonym Gene Names
IFN-alpha-1/13; LeIF D
UniProt Entry Name
IFNA1_HUMAN
NCBI Description
The protein encoded by this gene is produced by macrophages and has antiviral activity. This gene is intronless and the encoded protein is secreted. [provided by RefSeq, Sep 2011]
Uniprot Description
IFNA1/13: a cytokine that belongs to the alpha/beta interferon family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 9p22
Cellular Component: extracellular region; extracellular space
Molecular Function: cytokine activity; interferon-alpha/beta receptor binding
Biological Process: adaptive immune response; B cell differentiation; B cell proliferation; blood coagulation; cytokine and chemokine mediated signaling pathway; defense response to virus; humoral immune response; innate immune response; natural killer cell activation during immune response; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to exogenous dsRNA; T cell activation during immune response
Research Articles on IFNA1
Similar Products
Product Notes
The Human IFNA1 ifna1 (Catalog #AAA8244565) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8244565 ELISA Kit recognizes Human IFNA1. AAA Biotech's IFN alpha 1 can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (EIA). Researchers should empirically determine the suitability of the IFNA1 ifna1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IFN alpha 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
