Sheep ETFbeta ELISA Kit | ETFbeta elisa kit
Sheep ETFbeta (Electron Transfer Flavoprotein Beta Polypeptide) ELISA Kit
Gene Names
ETFBETA; electron transfer flavoprotein beta; ETFBETA; MWF20.14; MWF20_14
Reactivity
Sheep
Synonyms
ETFbeta; Sheep ETFbeta (Electron Transfer Flavoprotein Beta Polypeptide) ELISA Kit; ETFbeta elisa kit
Reactivity
Sheep
Specificity
This kit recognizes natural and recombinant Sheep ETFbeta. No significant cross-reactivity or interference between Sheep ETFbeta and analogues was observed.
Sequence Length
219
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
31.25-2000pg/mL
Sensitivity
Min: 18.75pg/mL; Max: 2000pg/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ETFbeta elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep ETFbeta concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ETFbeta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ETFbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ETFbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ETFbeta. You can calculate the concentration of ETFbeta in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ETFbeta. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ETFbeta and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ETFbeta, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of ETFbeta. You can calculate the concentration of ETFbeta in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,792 Da
NCBI Official Full Name
electron transfer flavoprotein subunit beta
NCBI Official Symbol
ETFBETA
NCBI Official Synonym Symbols
electron transfer flavoprotein beta; ETFBETA; MWF20.14; MWF20_14
NCBI Protein Information
electron transfer flavoprotein subunit beta
UniProt Protein Name
Electron transfer flavoprotein subunit beta, mitochondrial
UniProt Gene Name
ETFB
UniProt Synonym Gene Names
Beta-ETF
UniProt Entry Name
ETFB_ARATH
Uniprot Description
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (). Involved in leucine catabolism and in phytol degradation.
