Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear inhibitor of protein phosphatase 1 (PPP1R8) Standard Curve.)
Mouse Nuclear Inhibitor Of Protein Phosphatase 1 (PPP1R8) ELISA Kit | NIPP-1 elisa kit
Mouse Nuclear Inhibitor Of Protein Phosphatase 1 (PPP1R8) ELISA Kit
Gene Names
Ppp1r8; NIPP1; AU044684; 6330548N22Rik
Reactivity
Mouse
Synonyms
Nuclear Inhibitor Of Protein Phosphatase 1 (PPP1R8); Mouse Nuclear Inhibitor Of Protein Phosphatase 1 (PPP1R8) ELISA Kit; PPP1R8; ARD-1; ARD1; NIPP1; PRO2047; RNase E; activator of RNA decay; nuclear inhibitor of protein phosphatase-1; nuclear subunit of PP-1; protein phosphatase 1 regulatory inhibitor subunit 8; protein ph; NIPP-1 elisa kit
Reactivity
Mouse
Specificity
Mouse Nuclear inhibitor of protein phosphatase 1 (PPP1R8) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PPP1R8. No significant cross-reactivity or interference between Mouse PPP1R8 and analogues was observed.
Sequence Length
350
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear inhibitor of protein phosphatase 1 (PPP1R8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Nuclear inhibitor of protein phosphatase 1 (PPP1R8) Standard Curve.)
Related Product Information for NIPP-1 elisa kit
Description: This Mouse Nuclear inhibitor of protein phosphatase 1 (PPP1R8) ELISA Kit employs a two-site sandwich ELISA to quantitate PPP1R8 in samples. An antibody specific for PPP1R8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PPP1R8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPP1R8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPP1R8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Nuclear inhibitor of protein phosphatase 1, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.The 351-amino acid NIPP1 protein is a specific inhibitor of type 1 serine/threonine protein phosphatases. The human ARD1 amino acid sequence is virtually identical to the carboxy terminus of the bovine NIPP1 amino acid sequence.
Background: Nuclear inhibitor of protein phosphatase 1, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.The 351-amino acid NIPP1 protein is a specific inhibitor of type 1 serine/threonine protein phosphatases. The human ARD1 amino acid sequence is virtually identical to the carboxy terminus of the bovine NIPP1 amino acid sequence.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nuclear inhibitor of protein phosphatase 1 isoform 2
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 8
NCBI Official Symbol
Ppp1r8
NCBI Official Synonym Symbols
NIPP1; AU044684; 6330548N22Rik
NCBI Protein Information
nuclear inhibitor of protein phosphatase 1
UniProt Protein Name
Nuclear inhibitor of protein phosphatase 1
UniProt Gene Name
Ppp1r8
UniProt Synonym Gene Names
Nipp1; NIPP-1
UniProt Entry Name
PP1R8_MOUSE
Research Articles on NIPP-1
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Product Notes
The Mouse NIPP-1 ppp1r8 (Catalog #AAA9717308) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717308 ELISA Kit recognizes Mouse NIPP-1. It is sometimes possible for the material contained within the vial of "Nuclear Inhibitor Of Protein Phosphatase 1 (PPP1R8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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