Typical Testing Data/Standard Curve (for reference only)
Monkey Interleukin-4 receptor subunit alpha (IL4R) ELISA Kit | IL4R elisa kit
Monkey Interleukin-4 receptor subunit alpha (IL4R) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-4Ralpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-4Ralpha antibody and an IL-4Ralpha-HRP conjugate. The assay sample and buffer are incubated together with IL-4Ralpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-4Ralpha concentration since IL-4Ralpha from samples and IL-4Ralpha-HRP conjugate compete for the anti-IL-4Ralpha antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-4Ralpha from the sample, fewer sites are left to bind IL-4Ralpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-4Ralpha concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2012]
Uniprot Description
IL4R: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Belongs to the type I cytokine receptor family. Type 4 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 16p12.1-p11.2
Cellular Component: extracellular space; integral to plasma membrane; receptor complex
Molecular Function: protein binding; receptor signaling protein activity; interleukin-4 receptor activity
Biological Process: ovulation; positive regulation of T-helper 2 cell differentiation; negative regulation of T-helper 1 cell differentiation; production of molecular mediator of acute inflammatory response; response to estrogen stimulus; immune response; defense response to protozoan; signal transduction; regulation of cell proliferation; positive regulation of macrophage activation
Disease: Ige Responsiveness, Atopic; Human Immunodeficiency Virus Type 1, Susceptibility To
Research Articles on IL4R
Similar Products
Product Notes
The Monkey IL4R il4r (Catalog #AAA7226337) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7226337 ELISA Kit recognizes Monkey IL4R. It is sometimes possible for the material contained within the vial of "Interleukin-4 receptor subunit alpha (IL4R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
