IFI16 / Gamma-Interferon-Inducible Protein 16 Recombinant Protein | IFI16 recombinant protein

Human IFI16 / Gamma-Interferon-Inducible Protein 16 Recombinant Protein

• Gene Names: IFI16; PYHIN2; IFNGIP1
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: IFI16 / Gamma-Interferon-Inducible Protein 16; Human IFI16 / Gamma-Interferon-Inducible Protein 16 Recombinant Protein; Gamma-interferon-inducible protein 16; Ifi-16; Interferon-inducible myeloid differentiation transcriptional activator; IFNGIP1; IFI16 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 729

• Applicable Applications for IFI16 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Forms homooligomers; isoform 2 can self-associate or associate with isoform 1 or isoform 3. Interacts with TMEM173, AIM2, PYCARD and CASP1. Interacts with BRCA1, TP53, E2F1, RB1 and SP1. Interacts with MTA1.
• Entry Function: Binds double-stranded DNA. Binds preferentially to supercoiled DNA and cruciform DNA structures. Seems to be involved in transcriptional regulation. May function as a transcriptional repressor. Could have a role in the regulation of hematopoietic differentiation through activation of unknown target genes. Controls cellular proliferation by modulating the functions of cell cycle regulatory factors including p53/TP53 and the retinoblastoma protein. May be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. Seems to be involved in energy-level-dependent activation of the ATM/ AMPK/TP53 pathway coupled to regulation of autophagy. May be involved in regulation of TP53-mediated cell death also involving BRCA1. May be involved in the senescence of prostate epithelial cells. Involved in innate immune response by recognizing viral dsDNA in the cytosol and probably in the nucleus. After binding to viral DNA in the cytoplasm recruits TMEM173/STING and mediates the induction of IFN-beta. Has anti-inflammatory activity and inhibits the activation of the AIM2 inflammasome, probably via association with AIM2. Proposed to bind viral DNA in the nucleus, such as of Kaposi's sarcoma-associated herpesvirus, and to induce the formation of nuclear caspase-1-activating inflammasome formation via association with PYCARD. Inhibits replication of herpesviruses such as human cytomegalovirus (HCMV) probably by interfering with promoter recruitment of members of the Sp1 family of transcription factors. Necessary to activate the IRF3 signaling cascade during human herpes simplex virus 1 (HHV-1) infection and promotes the assembly of heterochromatin on herpesviral DNA and inhibition of viral immediate-early gene expression and replication. Involved in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 330864753
• NCBI GeneID: 3428
• NCBI Accession #: NP_001193496.1
• NCBI GenBank Nucleotide #: NP_001193496.1
• UniProt Accession #: Q16666
• NCBI Official Full Name: gamma-interferon-inducible protein 16 isoform 1
• NCBI Official Synonym Full Names: interferon gamma inducible protein 16
• NCBI Official Symbol: IFI16
• NCBI Official Synonym Symbols: PYHIN2; IFNGIP1
• NCBI Protein Information: gamma-interferon-inducible protein 16
• UniProt Protein Name: Gamma-interferon-inducible protein 16
• Protein Family: Gamma-interferon-inducible protein
• UniProt Gene Name: IFI16
• UniProt Synonym Gene Names: IFNGIP1; Ifi-16
• UniProt Entry Name: IF16_HUMAN

NCBI Description

This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]

Uniprot Description

IFI16: a nuclear protein that may function as a transcriptional repressor. Strongly induced by gamma interferon and, to a lesser extent, by alpha interferon. Binds double-stranded DNA and cell cycle regulatory factors including p53 and Rb. Loss of IFI16 activates p53 checkpoint through NBS1-DNAPK pathway. Inhibits cell growth in the Ras/Raf signaling pathway. May be involved in the senescence of prostate epithelial cells. Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast. Four alternatively spliced isoforms have been described. Isoforms-1, -2 and -3 can homo- and hetero-dimerize.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nuclear speck; cytosol; nucleus

Molecular Function: identical protein binding; protein binding; double-stranded DNA binding; transcription factor binding

Biological Process: positive regulation of cytokine production; transcription, DNA-dependent; monocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of DNA binding; cellular response to glucose starvation; cell proliferation; positive regulation of interleukin-1 beta production; negative regulation of viral genome replication; negative regulation of innate immune response; positive regulation of interferon type I production; autophagy; innate immune response; myeloid cell differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; regulation of autophagy; negative regulation of transcription, DNA-dependent; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; activation of innate immune response