Rabbit PLD1 Polyclonal Antibody | anti-PLD1 antibody

PLD1, ID (PLD1, Phosphatidylcholine-Specific Phospholipase D1, HPLD1)

Cat. Num. AAA646401

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: PLD1; Polyclonal Antibody; ID (PLD1; Phosphatidylcholine-Specific Phospholipase D1; HPLD1); Anti -PLD1; anti-PLD1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLD1. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-PLD1 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:1000; Immunohistochemistry (Paraffin) 10ug/ml
• Immunogen: Synthetic peptide corresponding to human PLD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-PLD1 antibody

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties.

Product Categories/Family for anti-PLD1 antibody

Antibodies; Abs to Enzymes; Phospholipase

NCBI and Uniprot Product Information

• NCBI GI #: 194272176
• NCBI GeneID: 5337
• NCBI Accession #: NP_001123553.1
• NCBI GenBank Nucleotide #: NM_001130081.2
• UniProt Accession #: Q13393
• Molecular Weight: 124,184 Da
• NCBI Official Full Name: phospholipase D1 isoform b
• NCBI Official Synonym Full Names: phospholipase D1, phosphatidylcholine-specific
• NCBI Official Symbol: PLD1
• NCBI Protein Information: phospholipase D1; choline phosphatase 1; phosphatidylcholine-hydrolyzing phospholipase D1
• UniProt Protein Name: Phospholipase D1
• Protein Family: Pyridoxal 4-dehydrogenase
• UniProt Gene Name: PLD1
• UniProt Synonym Gene Names: PLD 1; hPLD1
• UniProt Entry Name: PLD1_HUMAN

NCBI Description

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]

Uniprot Description

Function: Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic

By similarity.

Catalytic activity: A phosphatidylcholine + H2O = choline + a phosphatidate.

Enzyme regulation: Stimulated by phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate, activated by the phosphokinase C-alpha, by the ADP-ribosylation factor-1 (ARF-1), and to a lesser extent by GTP-binding proteins: RHO A, RAC-1 and CDC42. Inhibited by oleate.

Subunit structure: Interacts with PIP5K1B. Ref.7

Subcellular location: Cytoplasm › perinuclear region

By similarity. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side

By similarity. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side

By similarity. Late endosome membrane; Lipid-anchor; Cytoplasmic side

By similarity.

Tissue specificity: Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine. Ref.6

Sequence similarities: Belongs to the phospholipase D family.Contains 1 PH domain.Contains 2 PLD phosphodiesterase domains.Contains 1 PX (phox homology) domain.