Rat Phosphorylated Smad 2 (pSmad3) ELISA Kit | pSmad3 elisa kit

Rat Phosphorylated Smad 2 (pSmad3) ELISA Kit

Cat. Num. AAA269938

• Gene Names: Ppm1a; Pp2c1
• Reactivity: Rat
• Synonyms: Phosphorylated Smad 2 (pSmad3); Rat Phosphorylated Smad 2 (pSmad3) ELISA Kit; pSmad3 elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 382

• Samples: Rat Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Psmad2 Concentration
• Assay Type: Sandwich
• Detection Range: 5000 pg/ml-78 pg/ml
• Sensitivity: 20 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for pSmad3 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat pSmad2 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 8394012
• NCBI GeneID: 24666
• NCBI Accession #: NP_058734.1
• NCBI GenBank Nucleotide #: NM_017038.1
• Molecular Weight: 42,417 Da
• NCBI Official Full Name: protein phosphatase 1A
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1A
• NCBI Official Symbol: Ppm1a
• NCBI Official Synonym Symbols: Pp2c1
• NCBI Protein Information: protein phosphatase 1A; IA; PP2C-alpha; protein phosphatase IA; protein phosphatase 2C isoform alpha; protein phosphatase 1A, magnesium dependent, alpha isoform; Protein phosphatase type 1A (formely 2C) Mg-dependent alpha isoform; Protein phosphatase type 1A (formely 2C), Mg-dependent, alpha isoform
• UniProt Protein Name: Protein phosphatase 1A
• UniProt Gene Name: Ppm1a
• UniProt Synonym Gene Names: Pp2c1; Pppm1a; PP2C-alpha
• UniProt Entry Name: PPM1A_RAT

Uniprot Description

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Cellular Component: neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity; phosphoprotein phosphatase activity

Biological Process: positive regulation of protein export from nucleus; N-terminal protein myristoylation; Wnt receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; dephosphorylation; positive regulation of transcription, DNA-dependent; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of NF-kappaB import into nucleus; negative regulation of transforming growth factor beta receptor signaling pathway; protein amino acid dephosphorylation; positive regulation of Wnt receptor signaling pathway; negative regulation of BMP signaling pathway