ID2 recombinant protein

ID2, 1-134aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205123

• Gene Names: ID2; GIG8; ID2A; ID2H; bHLHb26
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: ID2; 1-134aa; Human; His tag; E Coli (Denatured); Inhibitor of DNA binding 2; bHLHb26; GIG8; ID2A; ID2H; MGC26389; ID2 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol 0.4M urea
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVS KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQASR TPLTTLNTDI SILSLQASEF PSELMSNDSK ALCG
• Sequence Length: 134

• Applicable Applications for ID2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ID2 recombinant protein

ID2 belongs to the inhibitor of DNA binding (ID) family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the ID family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. Recombinant human ID2 protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for ID2 recombinant protein

Epigenomics

References

Hara E. et al. (1994) J Biol Chem. 269:2139-2145.; Langlands Ket al. (1997) J Biol Chem. 272:19785-19793.;

NCBI and Uniprot Product Information

• NCBI GI #: 31982933
• NCBI GeneID: 3398
• NCBI Accession #: NP_002157
• NCBI GenBank Nucleotide #: NM_002166.4
• UniProt Accession #: Q02363
• Molecular Weight: 17.0 kDa (154aa)
• NCBI Official Full Name: DNA-binding protein inhibitor ID-2
• NCBI Official Synonym Full Names: inhibitor of DNA binding 2
• NCBI Official Symbol: ID2
• NCBI Official Synonym Symbols: GIG8; ID2A; ID2H; bHLHb26
• NCBI Protein Information: DNA-binding protein inhibitor ID-2
• UniProt Protein Name: DNA-binding protein inhibitor ID-2
• Protein Family: DNA-binding protein inhibitor
• UniProt Gene Name: ID2
• UniProt Synonym Gene Names: BHLHB26; bHLHb26

NCBI Description

The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Aug 2011]

Uniprot Description

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.