ICOS / AILIM / CD278 recombinant protein

Recombinant Mouse ICOS / AILIM / CD278 Protein (Fc tag)

Cat. Num. AAA2546967

• Gene Names: Icos; H4; CCLP; AILIM; CRP-1; Ly115
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ICOS / AILIM / CD278; Recombinant Mouse ICOS / AILIM / CD278 Protein (Fc tag); CH29-120A16.4; AILIM; CCLP; CRP-1; H4; Ly115; ICOS / AILIM / CD278 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 200

• Application Notes: The recombinant mouse ICOS/Fc is a disulfide-linked homodimer. The reduced monomer consists of 363 amino acids and has a predicted molecular mass of 40.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmICOS/Fc monomer is approximately 40-45 kDa.
• Predicted N Terminal: Glu 21
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for ICOS / AILIM / CD278 recombinant protein

Background: Inducible costimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) is a cell-surface receptor, and belongs to the CD28 family of immune costimulatory receptors consisting of CD28, CTLA-4 and PD-1. The interaction of B7-H2/ICOS plays a critical role in Th cell differentiation, T-B cell interactions which is essential for germinal center formation, and humoral immune responses, and as well as the production of cytokine IL-4. In addition, ICOS is more potent in the induction of IL-10 production, a cytokine important for suppressive function of T regulatory cells. The B7-1/B7-2--CD28/CTLA-4 and ICOS-B7RP-1 pathway provides key second signals that can regulate the activation, inhibition and fine-tuning of T-lymphocyte responses. ICOS stimulates both Th1 and Th2 cytokine production but may have a preferential role in Th2 cell development. Moreover, The B7-1/B7-2-CD28/CTLA-4 and ICOS-B7RP-1 pathway has been suggested of being involved in the development of airway inflammation and airway hyperresponsiveness.

Description: A DNA sequence encoding the mouse ICOS (Q9WVS0) extracellular domain (Met 1-Leu 142) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 224809335
• NCBI GeneID: 54167
• NCBI Accession #: NP_059508.2
• NCBI GenBank Nucleotide #: NM_017480.2
• UniProt Accession #: Q9WVS0; Q9JL17
• Molecular Weight: 22,709 Da
• NCBI Official Full Name: inducible T-cell costimulator
• NCBI Official Synonym Full Names: inducible T cell co-stimulator
• NCBI Official Symbol: Icos
• NCBI Official Synonym Symbols: H4; CCLP; AILIM; CRP-1; Ly115
• NCBI Protein Information: inducible T-cell costimulator
• UniProt Protein Name: Inducible T-cell costimulator
• UniProt Gene Name: Icos
• UniProt Synonym Gene Names: Ailim; CCLP; CRP-1

Uniprot Description

ICOS: Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up- regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up- regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre- activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes. Defects in ICOS are the cause of immunodeficiency common variable type 1 (CVID1). CVID1 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be lo. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1 C2|1 30.6 cM

Cellular Component: external side of plasma membrane; integral to plasma membrane

Molecular Function: protein binding

Biological Process: cell-cell adhesion; T cell costimulation; T cell tolerance induction