Mouse anti-Human, Mouse SLC2A4 Monoclonal Antibody | anti-SLC2A4 antibody

Anti-SLC2A4 Mouse mAb

Cat. Num. AAA476382

• Gene Names: SLC2A4; GLUT4
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
• Synonyms: SLC2A4; Monoclonal Antibody; Anti-SLC2A4 Mouse mAb; GLUT4; anti-SLC2A4 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3G10A3
• Form/Format: Ascitic fluid containing 0.03% sodium azide.
• Sequence Length: 509

• Applicable Applications for anti-SLC2A4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Immunogen: Purified recombinant fragment of human SLC2A4 (AA: 224-353) expressed in E Coli.

Western Blot (WB)

(Western blot analysis using SLC2A4 mAb against human SLC2A4 recombinant protein. (Expected MW is 39.9 kDa))

Western Blot (WB)

(Western blot analysis using SLC2A4 mAb against HEK293 (1) and SLC2A4 (AA)

Western Blot (WB)

(Western blot analysis using SLC2A4 mouse mAb against NIH3T3 (1), 3T3L1 (2), MCF-7 (4) cell lysate and Mouse heart (3) tissue lysate.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cells using SLC2A4 mouse mAb (green). Blue)

Immunofluorescence (IF)

(Immunofluorescence analysis of HepG2 cells using SLC2A4 mouse mAb (green). Blue)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of HeLa cells using SLC2A4 mouse mAb (green) and negative control (purple).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 mouse mAb with DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.)

Related Product Information for anti-SLC2A4 antibody

Entrez Summary: This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

NCBI and Uniprot Product Information

• NCBI GI #: 4507011
• NCBI GeneID: 6517
• NCBI Accession #: NP_001033.1
• NCBI GenBank Nucleotide #: NM_001042.2
• UniProt Accession #: P14672; Q05BQ3; Q14CX2
• Molecular Weight: 54.8
• NCBI Official Full Name: solute carrier family 2, facilitated glucose transporter member 4
• NCBI Official Synonym Full Names: solute carrier family 2 member 4
• NCBI Official Symbol: SLC2A4
• NCBI Official Synonym Symbols: GLUT4
• NCBI Protein Information: solute carrier family 2, facilitated glucose transporter member 4
• UniProt Protein Name: Solute carrier family 2, facilitated glucose transporter member 4
• Protein Family: SLC2A4 regulator
• UniProt Gene Name: SLC2A4
• UniProt Synonym Gene Names: GLUT4; GLUT-4

NCBI Description

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Uniprot Description

GLUT4: an integral membrane facilitative glucose transporter. One of 13 members of the human equilibrative glucose transport protein family. Its surface expression is regulated by insulin. Has two internalization sequences, a dileucine repeat present in the C-terminus and a FxxY motif in the amino-terminal end. Associates with an intracellular tubulo-vesicular compartment under low plasma conditions. The binding of insulin to the insulin receptor (a typosine kinase) leads to the rapid translocation of GLUT4 to the cell surface, increasing cellular glucose transport activity. Its translocation to the plasma membrane is also stimulated by exercise, but independent from insulin signaling. This insulin-independent pathway may be regulated by AMPK.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: clathrin-coated vesicle; endomembrane system; external side of plasma membrane; membrane; perinuclear region of cytoplasm; plasma membrane; vesicle membrane

Molecular Function: D-glucose transmembrane transporter activity; glucose transmembrane transporter activity; protein binding

Biological Process: carbohydrate metabolic process; cellular response to insulin stimulus; glucose homeostasis; glucose transport

Disease: Diabetes Mellitus, Noninsulin-dependent