Human Ribonuclease L (RNASEL) RTU ELISA Kit | RNASEL rtu elisa kit

Human Ribonuclease L (RNASEL) ELISA Kit

Cat. Num. AAA4502266

• Gene Names: RNASEL; RNS4; PRCA1
• Reactivity: Human
• Synonyms: Ribonuclease L (RNASEL); Human Ribonuclease L (RNASEL) ELISA Kit; Rnase-L; RNS4; PRCA1; 2'; 5'-Oligoisoadenylate Synthetase-Dependent; Prostate Cancer 1; 2-5A-dependent ribonuclease; RNASEL rtu elisa kit

• Reactivity: Human
• Sequence Length: 741

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 7.3pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNASEL rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RNASEL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to RNASEL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RNASEL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of RNASEL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10863929
• NCBI GeneID: 6041
• NCBI Accession #: NP_066956.1
• NCBI GenBank Nucleotide #: NM_021133.3
• UniProt Accession #: Q05823; Q5W0L2; Q6AI46
• Molecular Weight: 73,416 Da
• NCBI Official Full Name: 2-5A-dependent ribonuclease
• NCBI Official Synonym Full Names: ribonuclease L
• NCBI Official Symbol: RNASEL
• NCBI Official Synonym Symbols: RNS4; PRCA1
• NCBI Protein Information: 2-5A-dependent ribonuclease
• UniProt Protein Name: 2-5A-dependent ribonuclease
• Protein Family: 2-5A-dependent ribonuclease
• UniProt Gene Name: RNASEL
• UniProt Synonym Gene Names: RNS4; 2-5A-dependent RNase; RNase L

NCBI Description

This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]

Uniprot Description

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979). Might play a central role in the regulation of mRNA turnover (PubMed:11585831). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.