Human Ribonuclease L (RNASEL) ELISA Kit | RNASEL elisa kit

Human Ribonuclease L (RNASEL) ELISA Kit

Cat. Num. AAA2704946

• Gene Names: RNASEL; RNS4; PRCA1
• Reactivity: Human
• Synonyms: Ribonuclease L (RNASEL); Human Ribonuclease L (RNASEL) ELISA Kit; Rnase-L; RNS4; PRCA1; 2'; 5'-Oligoisoadenylate Synthetase-Dependent; Prostate Cancer 1; 2-5A-dependent ribonuclease; RNASEL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ribonuclease L (RNASEL).; No significant cross-reactivity or interference between Ribonuclease L (RNASEL) and analogues was observed.
• Sequence Length: 652

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease L (RNASEL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease L (RNASEL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNASEL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease L (RNASEL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease L (RNASEL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease L (RNASEL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease L (RNASEL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 89365969
• NCBI GeneID: 6041
• UniProt Accession #: Q05823; Q5W0L2; Q6AI46
• Molecular Weight: 73,416 Da
• NCBI Official Full Name: Ribonuclease L
• NCBI Official Synonym Full Names: ribonuclease L
• NCBI Official Symbol: RNASEL
• NCBI Official Synonym Symbols: RNS4; PRCA1
• NCBI Protein Information: 2-5A-dependent ribonuclease
• UniProt Protein Name: 2-5A-dependent ribonuclease
• Protein Family: 2-5A-dependent ribonuclease
• UniProt Gene Name: RNASEL
• UniProt Synonym Gene Names: RNS4; 2-5A-dependent RNase; RNase L

NCBI Description

This gene encodes a component of the interferon-regulated 2-5A system that functions in the antiviral and antiproliferative roles of interferons. Mutations in this gene have been associated with predisposition to prostate cancer and this gene is a candidate for the hereditary prostate cancer 1 (HPC1) allele. [provided by RefSeq, Jul 2008]

Uniprot Description

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979). Might play a central role in the regulation of mRNA turnover (PubMed:11585831). Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.