Human Protein Kinase B Gamma (PKBg) RTU ELISA Kit | PKBg rtu elisa kit

Human Protein Kinase B Gamma (PKBg) ELISA Kit

Cat. Num. AAA4501862

• Gene Names: AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• Reactivity: Human
• Synonyms: Protein Kinase B Gamma (PKBg); Human Protein Kinase B Gamma (PKBg) ELISA Kit; PKBG; PRKB-G; AKT3; RAC-PK-Gamma; RAC-Gamma; STK2; RAC-Gamma Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 3; PKBg rtu elisa kit

• Reactivity: Human
• Sequence Length: 465

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 1.56-100ng/mL
• Sensitivity: 0.56ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKBg rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDPN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PDPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDPN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 332078559
• NCBI GeneID: 10000
• NCBI Accession #: NP_001193658.1
• NCBI GenBank Nucleotide #: NM_001206729.1
• UniProt Accession #: Q9Y243; Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5
• Molecular Weight: 54,032 Da
• NCBI Official Full Name: RAC-gamma serine/threonine-protein kinase isoform 2
• NCBI Official Synonym Full Names: AKT serine/threonine kinase 3
• NCBI Official Symbol: AKT3
• NCBI Official Synonym Symbols: MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• NCBI Protein Information: RAC-gamma serine/threonine-protein kinase
• UniProt Protein Name: RAC-gamma serine/threonine-protein kinase
• UniProt Gene Name: AKT3
• UniProt Synonym Gene Names: PKBG; PKB gamma

NCBI Description

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.