Rabbit anti-Human Fas Associating Death Domain Containing Protein (FADD) Polyclonal Antibody | anti-FADD antibody

Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD)

Cat. Num. AAA2016763

• Gene Names: FADD; GIG3; MORT1
• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Fas Associating Death Domain Containing Protein (FADD); Polyclonal Antibody; Polyclonal Antibody to Fas Associating Death Domain Containing Protein (FADD); anti-FADD antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against FADD. It has been selected for its ability to recognize FADD in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SSSLSSSEL TELKFLCLGR VGKRKLERVQ SGLDLFSMLL EQNDLEPGHT ELLRELLASL RRHDLLRRVD DFEAGAAAGA APGEEDLCAA FNVICDNVGK DWRRLARQLK VSDTKIDSIE DRYPRNLTER VRESLRIWKN
• Sequence Length: 193

• Applicable Applications for anti-FADD antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant FADD (Ser12~Glu206) expressed in E.coli.
• Cross Reactivity: Human, Mouse
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2081985
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Lane1: Human Serum; Lane2: Mouse RAW 264.7 Cells.)

Western Blot (WB)

(Western Blot: Sample: Recombinant FADD, Human.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Cancer Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505229
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815.1
• NCBI GenBank Nucleotide #: NM_003824.3
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: 23,279 Da
• NCBI Official Full Name: FAS-associated death domain protein
• NCBI Official Synonym Full Names: Fas associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: FAS-associated death domain protein
• UniProt Protein Name: FAS-associated death domain protein
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

FADD: an adaptor molecule that mediates apoptosis. Recruited through its C-terminal death domain by Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TRAIL-receptor, participating in the death signaling initiated by these receptors. Interaction with the receptors unmasks the N-terminal effector domain of this protein, which recruits caspase-8, and thereby activates the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.

Protein type: Adaptor/scaffold; Apoptosis

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: CD95 death-inducing signaling complex; cell body; cytoplasm; cytosol; membrane raft; neuron projection; nucleus; plasma membrane

Molecular Function: caspase binding; death receptor binding; identical protein binding; protease binding; protein binding; protein complex binding; receptor serine/threonine kinase binding; tumor necrosis factor receptor binding; tumor necrosis factor receptor superfamily binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; apoptosis; behavioral response to cocaine; cell surface receptor signaling pathway; death-inducing signaling complex assembly; defense response to virus; induction of apoptosis via death domain receptors; innate immune response; lymph node development; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of apoptosis; positive regulation of CD8-positive, alpha-beta cytotoxic T cell extravasation; positive regulation of extrinsic apoptotic signaling pathway; positive regulation of extrinsic apoptotic signaling pathway via death domain receptors; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon-gamma production; positive regulation of interleukin-8 production; positive regulation of macrophage differentiation; positive regulation of proteolysis; positive regulation of T cell mediated cytotoxicity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; regulation of extrinsic apoptotic signaling pathway via death domain receptors; response to morphine; spleen development; T cell differentiation in thymus; T cell homeostasis; thymus development; toll-like receptor 3 signaling pathway; TRAIL-activated apoptotic signaling pathway; viral process

Disease: Infections, Recurrent, With Encephalopathy, Hepatic Dysfunction, And Cardiovascular Malformations