Human Protein Kinase B Gamma (PKBg) ELISA Kit | PKBg elisa kit

Human Protein Kinase B Gamma (PKBg) ELISA Kit

Cat. Num. AAA2701708

• Gene Names: AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• Reactivity: Human
• Synonyms: Protein Kinase B Gamma (PKBg); Human Protein Kinase B Gamma (PKBg) ELISA Kit; PKBG; PRKB-G; AKT3; RAC-PK-Gamma; RAC-Gamma; STK2; RAC-Gamma Serine/Threonine-Protein Kinase; V-Akt Murine Thymoma Viral Oncogene Homolog 3; PKBg elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Kinase B Gamma (PKBg).; No significant cross-reactivity or interference between Protein Kinase B Gamma (PKBg) and analogues was observed.
• Sequence Length: 479

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.60ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Kinase B Gamma (PKBg) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Kinase B Gamma (PKBg) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKBg elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase B Gamma (PKBg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Kinase B Gamma (PKBg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase B Gamma (PKBg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase B Gamma (PKBg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4757579
• NCBI GeneID: 10000
• UniProt Accession #: Q9Y243; Q0VAA6; Q5VTI1; Q5VTI2; Q96QV3; Q9UFP5
• Molecular Weight: 54,032 Da
• NCBI Official Full Name: protein kinase B gamma
• NCBI Official Synonym Full Names: AKT serine/threonine kinase 3
• NCBI Official Symbol: AKT3
• NCBI Official Synonym Symbols: MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
• NCBI Protein Information: RAC-gamma serine/threonine-protein kinase
• UniProt Protein Name: RAC-gamma serine/threonine-protein kinase
• UniProt Gene Name: AKT3
• UniProt Synonym Gene Names: PKBG; PKB gamma

NCBI Description

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.