Testing Data
(Detection limit for recombinant GST tagged NAE1 is 0.3 ng/ml as a capture antibody.)
Mouse NAE1 Monoclonal Antibody | anti-NAE1 antibody
NAE1 (NEDD8 Activating Enzyme E1 Subunit 1, A-116A10.1, APPBP1, HPP1, ula-1) (HRP)
Gene Names
NAE1; HPP1; ula-1; APPBP1; A-116A10.1
Applications
Western Blot
Purity
Purified
Synonyms
NAE1; Monoclonal Antibody; NAE1 (NEDD8 Activating Enzyme E1 Subunit 1; A-116A10.1; APPBP1; HPP1; ula-1) (HRP); NEDD8 Activating Enzyme E1 Subunit 1; ula-1; anti-NAE1 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
2E9-D12
Specificity
Recognizes NAE1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Sequence Length
534
Applicable Applications for anti-NAE1 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
NAE1 (AAH00480, 1aa-534aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Conjugate
HRP
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Detection limit for recombinant GST tagged NAE1 is 0.3 ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged NAE1 is 0.3 ng/ml as a capture antibody.)
Western Blot (WB)
(Western Blot analysis of NAE1 expression in transfected 293T cell line by NAE1 monoclonal antibody (M02), clone 2E9-D12.Lane 1: NAE1 transfected lysate (Predicted MW: 60.2 KDa).Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NAE1 expression in transfected 293T cell line by NAE1 monoclonal antibody (M02), clone 2E9-D12.Lane 1: NAE1 transfected lysate (Predicted MW: 60.2 KDa).Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-NAE1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
NEDD8 activating enzyme E1 subunit 1
NCBI Official Synonym Full Names
NEDD8 activating enzyme E1 subunit 1
NCBI Official Symbol
NAE1
NCBI Official Synonym Symbols
HPP1; ula-1; APPBP1; A-116A10.1
NCBI Protein Information
NEDD8-activating enzyme E1 regulatory subunit
Protein Family
NCBI Description
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Research Articles on NAE1
Similar Products
Product Notes
The NAE1 (Catalog #AAA6182576) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NAE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NAE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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