PLA2G2A recombinant protein

PLA2G2A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120723

• Gene Names: PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• Purity: >97% as determined by SDS-PAGE
• Synonyms: PLA2G2A; PLA2G2A Protein; Human; Recombinant (His Tag); Human PLA2G2A/PLA2B Protein (His Tag); MOM1 Protein; PLA2 Protein; PLA2B Protein; PLA2L Protein; PLA2S Protein; PLAS1 Protein; sPLA2 Protein; phospholipase A2; group IIA (platelets; synovial fluid); PLA2G2A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys144

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asn 21
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human PLA2G2A (NP_000291.1)(Met 1-Cys 144) was expressed, with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PLA2G2A recombinant protein

Background: Phospholipase A2, membrane associated, also known as Phosphatidylcholine 2-acylhydrolase 2A, Group IIA phospholipase A2, Non-pancreatic secretory phospholipase A2 and PLA2G2A, is a peripheral membrane protein that belongs to the phospholipase A2 family. PLA2G2A is found in many cells and also extracellularly. The membrane-bound and secreted forms of PLA2G2A are identical. PLA2G2A has been proposed to play a role in anti-bacterial defense, inflammation and eicosanoid generation, in clearance of apoptotic cells, and in the Wnt signaling pathway. PLA2G2A is thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. PLA2G2A catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G2A might be a factor in human colorectal tumorigenesis.

References

Praml,C. et al., 1998, Oncogene. 17 (15):2009-12. Fijneman,R.J. et al., 2008,Front Biosci 13 :4144-74. Fijneman,R.J. et al., 2009, Cell Oncol 31 (5):345-56.

NCBI and Uniprot Product Information

• NCBI GI #: 4505849
• NCBI GeneID: 5320
• NCBI Accession #: NP_000291.1
• NCBI GenBank Nucleotide #: NM_000300.3
• UniProt Accession #: P14555; Q6DN24; Q6IBD9; Q9UCD2; A8K5I7
• Molecular Weight: 16,083 Da
• NCBI Official Full Name: phospholipase A2, membrane associated
• NCBI Official Synonym Full Names: phospholipase A2, group IIA (platelets, synovial fluid)
• NCBI Official Symbol: PLA2G2A
• NCBI Official Synonym Symbols: MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2
• NCBI Protein Information: phospholipase A2, membrane associated; GIIC sPLA2; NPS-PLA2; group IIA phospholipase A2; non-pancreatic secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A
• UniProt Protein Name: Phospholipase A2, membrane associated
• Protein Family: Phospholipase
• UniProt Gene Name: PLA2G2A
• UniProt Synonym Gene Names: PLA2B; PLA2L; RASF-A; NPS-PLA2
• UniProt Entry Name: PA2GA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

Uniprot Description

PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family.

Protein type: Lipid Metabolism - arachidonic acid; Secreted, signal peptide; Vesicle; Endoplasmic reticulum; Phospholipase; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Lipid Metabolism - alpha-linolenic acid; Mitochondrial; Secreted; Lipid Metabolism - linoleic acid

Chromosomal Location of Human Ortholog: 1p35

Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule

Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding

Biological Process: defense response to Gram-positive bacterium; phosphatidic acid metabolic process; somatic stem cell maintenance; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; positive regulation of inflammatory response; negative regulation of epithelial cell proliferation

Disease: Colorectal Cancer