Human Non POU Domain Containing Octamer Binding Protein (NONO) RTU ELISA Kit | NONO rtu elisa kit

Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit

Cat. Num. AAA4501401

• Gene Names: NONO; P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
• Reactivity: Human
• Synonyms: Non POU Domain Containing Octamer Binding Protein (NONO); Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit; P54; NMT55; NRB54; P54NRB; Nuclear RNA-Binding Protein 54-kD; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex; 52 kDa subunit; NONO rtu elisa kit

• Reactivity: Human
• Sequence Length: 471

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.126ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NONO rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NONO. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NONO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NONO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NONO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224028244
• NCBI GeneID: 4841
• NCBI Accession #: NP_001138880.1
• NCBI GenBank Nucleotide #: NM_001145408.1
• UniProt Accession #: Q15233; O00201; P30807; Q12786; Q9BQC5; B7Z4C2; D3DVV4; F5GYZ3
• Molecular Weight: 43,866 Da
• NCBI Official Full Name: non-POU domain-containing octamer-binding protein isoform 1
• NCBI Official Synonym Full Names: non-POU domain containing octamer binding
• NCBI Official Symbol: NONO
• NCBI Official Synonym Symbols: P54; NMT55; NRB54; MRXS34; P54NRB; PPP1R114
• NCBI Protein Information: non-POU domain-containing octamer-binding protein
• UniProt Protein Name: Non-POU domain-containing octamer-binding protein
• Protein Family: Non-POU domain-containing octamer-binding protein
• UniProt Gene Name: NONO
• UniProt Synonym Gene Names: NRB54; NonO protein; p54nrb

NCBI Description

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

Uniprot Description

DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Important for the functional organization of GABAergic synapses. Plays a specific and important role in the regulation of synaptic RNAs and GPHN/gephyrin scaffold structure, through the regulation of GABRA2 transcript. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway (PubMed:28712728).