Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit | NONO elisa kit

Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit

Cat. Num. AAA2000052

• Gene Names: NONO; P54; NMT55; NRB54; P54NRB; PPP1R114
• Reactivity: Human
• Synonyms: Non POU Domain Containing Octamer Binding Protein (NONO); Human Non POU Domain Containing Octamer Binding Protein (NONO) ELISA Kit; P54; NMT55; NRB54; P54NRB; Nuclear RNA-Binding Protein 54-kD; 54 kDa nuclear RNA- and DNA-binding protein; DNA-binding p52/p100 complex; 52 kDa subunit; NONO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Non POU Domain Containing Octamer Binding Protein (NONO).; No significant cross-reactivity or interference between Non POU Domain Containing Octamer Binding Protein (NONO) and analogues was observed.
• Sequence Length: 471

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.056ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Non POU Domain Containing Octamer Binding Protein (NONO) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Non POU Domain Containing Octamer Binding Protein (NONO) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NONO elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Non POU Domain Containing Octamer Binding Protein (NONO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Non POU Domain Containing Octamer Binding Protein (NONO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non POU Domain Containing Octamer Binding Protein (NONO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NONO elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 224028246
• NCBI GeneID: 4841
• NCBI Accession #: NP_001138881.1
• NCBI GenBank Nucleotide #: NM_001145409.1
• UniProt Accession #: Q15233; O00201; P30807; Q12786; Q9BQC5; B7Z4C2; D3DVV4; F5GYZ3
• Molecular Weight: 43,866 Da
• NCBI Official Full Name: non-POU domain-containing octamer-binding protein isoform 1
• NCBI Official Synonym Full Names: non-POU domain containing, octamer-binding
• NCBI Official Symbol: NONO
• NCBI Official Synonym Symbols: P54; NMT55; NRB54; P54NRB; PPP1R114
• NCBI Protein Information: non-POU domain-containing octamer-binding protein
• UniProt Protein Name: Non-POU domain-containing octamer-binding protein
• Protein Family: Non-POU domain-containing octamer-binding protein
• UniProt Gene Name: NONO
• UniProt Synonym Gene Names: NRB54; NonO protein; p54nrb
• UniProt Entry Name: NONO_HUMAN

NCBI Description

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

Uniprot Description

NONO: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription. Together with PSPC1, required for the formation of nuclear paraspeckles. A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.

Protein type: RNA-binding; Nuclear receptor co-regulator; Nucleolus; RNA splicing

Chromosomal Location of Human Ortholog: Xq13.1

Cellular Component: nucleoplasm; nuclear matrix; membrane; nucleolus; nuclear speck; paraspeckles; nucleus

Molecular Function: identical protein binding; protein binding; nucleotide binding

Biological Process: circadian rhythm; transcription, DNA-dependent; RNA splicing; DNA repair; regulation of circadian rhythm; negative regulation of transcription, DNA-dependent; mRNA processing; DNA recombination