Western Blot (WB)
(TRUB1 Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the TRUB1 antibody detected the TRUB1 protein (arrow).)
Rabbit anti-Human TRUB1 Polyclonal Antibody | anti-Trub1 antibody
TRUB1, ID (TRUB1, PUS4, Probable tRNA pseudouridine synthase 1)
Gene Names
Trub1; C76870; AI448235; BB118943; 2610009I02Rik; 9030425C13Rik
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TRUB1; Polyclonal Antibody; ID (TRUB1; PUS4; Probable tRNA pseudouridine synthase 1); Anti -TRUB1; anti-Trub1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Trub1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
TRUB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 159-188 amino acids from the Central region of human TRUB1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TRUB1 Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the TRUB1 antibody detected the TRUB1 protein (arrow).)
Western Blot (WB)
(TRUB1 Antibody (Center) western blot analysis in uterus tumor cell line lysates (35ug/lane).This demonstrates the TRUB1 antibody detected the TRUB1 protein (arrow).)
Immunohistochemistry (IHC)
(TRUB1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRUB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(TRUB1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRUB1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-Trub1 antibody
Pseudouridine is an abundant component of rRNAs and tRNAs and is enzymatically generated by isomerization of uridine by pseudouridine synthase (Zucchini et al., 2003 [PubMed 12736709]).
Product Categories/Family for anti-Trub1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,348 Da
NCBI Official Full Name
Trub1 protein, partial
NCBI Official Synonym Full Names
TruB pseudouridine (psi) synthase homolog 1 (E. coli)
NCBI Official Symbol
Trub1
NCBI Official Synonym Symbols
C76870; AI448235; BB118943; 2610009I02Rik; 9030425C13Rik
NCBI Protein Information
probable tRNA pseudouridine synthase 1
UniProt Protein Name
Probable tRNA pseudouridine synthase 1
Protein Family
UniProt Gene Name
Trub1
UniProt Entry Name
TRUB1_MOUSE
Uniprot Description
Function: May be responsible for synthesis of pseudouridine from uracil in transfer RNAs
By similarity.
Catalytic activity: tRNA uridine = tRNA pseudouridine.
Sequence similarities: Belongs to the pseudouridine synthase TruB family.
Similar Products
Product Notes
The Trub1 trub1 (Catalog #AAA646579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRUB1, ID (TRUB1, PUS4, Probable tRNA pseudouridine synthase 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRUB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the Trub1 trub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRUB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
