Rat Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit | SFRP1 elisa kit

Rat Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

Cat. Num. AAA2705953

• Gene Names: Sfrp1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• Reactivity: Rat
• Synonyms: Secreted Frizzled Related Protein 1 (SFRP1); Rat Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit; FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2; SFRP1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 1 (SFRP1).; No significant cross-reactivity or interference between Secreted Frizzled Related Protein 1 (SFRP1) and analogues was observed.
• Sequence Length: 314

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secreted Frizzled Related Protein 1 (SFRP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secreted Frizzled Related Protein 1 (SFRP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFRP1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 1 (SFRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 1 (SFRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 65301472
• NCBI GeneID: 20377
• NCBI Accession #: NP_038862.2
• NCBI GenBank Nucleotide #: NM_013834.3
• UniProt Accession #: Q8C4U3; O08861; Q505A2; Q8R1J4
• Molecular Weight: 35,413 Da
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled-related protein 1
• NCBI Official Symbol: Sfrp1
• NCBI Official Synonym Symbols: sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik
• NCBI Protein Information: secreted frizzled-related protein 1
• UniProt Protein Name: Secreted frizzled-related protein 1
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: Sfrp1
• UniProt Synonym Gene Names: sFRP-1

Uniprot Description

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi (). Inhibits WNT1/WNT4-mediated TCF-dependent transcription ().