<
Western Blot (WB)
(DPP3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPP3 antibody detected the DPP3 protein (arrow).)
Rabbit anti-Human DPP3 Polyclonal Antibody | anti-DPP3 antibody
DPP3, CT (DPP3, Dipeptidyl peptidase 3, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase III) (APC)
Gene Names
DPP3; DPPIII
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DPP3; Polyclonal Antibody; CT (DPP3; Dipeptidyl peptidase 3; Dipeptidyl aminopeptidase III; Dipeptidyl arylamidase III; Dipeptidyl peptidase III) (APC); Dipeptidyl peptidase III; anti-DPP3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-DPP3 antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IF: 1:10-50
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 590-620 amino acids from the C-terminal region of human DPP3.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(DPP3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPP3 antibody detected the DPP3 protein (arrow).)
Western Blot (WB)
(DPP3 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPP3 antibody detected the DPP3 protein (arrow).)
Immunohistochemistry (IHC)
(DPP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DPP3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DPP3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DPP3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DPP3 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DPP3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of DPP3 Antibody (C-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Related Product Information for anti-DPP3 antibody
This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternate transcriptional splice variants have been characterized.
Product Categories/Family for anti-DPP3 antibody
References
Shukla, A.A., et al. FEBS J. 277(8):1861-1875(2010). Salopek-Sondi, B., et al. Biol. Chem. 389(2):163-167(2008). Gevaert, K., et al. Proteomics 5(14):3589-3599(2005). Abramic, M., et al. Int. J. Biochem. Cell Biol. 36(3):434-446(2004). Hillman, R.T., et al. Genome Biol. 5 (2), R8 (2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,658 Da
NCBI Official Full Name
dipeptidyl peptidase 3 isoform 1
NCBI Official Synonym Full Names
dipeptidyl-peptidase 3
NCBI Official Symbol
DPP3
NCBI Official Synonym Symbols
DPPIII
NCBI Protein Information
dipeptidyl peptidase 3
UniProt Protein Name
Dipeptidyl peptidase 3
UniProt Gene Name
DPP3
UniProt Synonym Gene Names
DPP III
UniProt Entry Name
DPP3_HUMAN
NCBI Description
This gene encodes a protein that is a member of the M49 family of metallopeptidases. This cytoplasmic protein binds a single zinc ion with its zinc-binding motif (HELLGH) and has post-proline dipeptidyl aminopeptidase activity, cleaving Xaa-Pro dipeptides from the N-termini of proteins. Increased activity of this protein is associated with endometrial and ovarian cancers. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2012]
Research Articles on DPP3
Similar Products
Product Notes
The DPP3 dpp3 (Catalog #AAA6293346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DPP3, CT (DPP3, Dipeptidyl peptidase 3, Dipeptidyl aminopeptidase III, Dipeptidyl arylamidase III, Dipeptidyl peptidase III) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPP3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IF: 1:10-50 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DPP3 dpp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
