Human Interleukin 28A (IL28A) RTU ELISA Kit | IL28A rtu elisa kit

Human Interleukin 28A (IL28A) ELISA Kit

Cat. Num. AAA4500325

• Gene Names: IFNL2; IL28A; IL-28A
• Reactivity: Human
• Synonyms: Interleukin 28A (IL28A); Human Interleukin 28A (IL28A) ELISA Kit; IFNL2; Interferon; Lambda 2; Cytokine Zcyto20; IL28A rtu elisa kit

• Reactivity: Human
• Sequence Length: 200

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.4pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL28A rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL28A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to IL28A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IL28A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IL28A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 26024321
• NCBI GeneID: 282616
• NCBI Accession #: NP_742150.1
• NCBI GenBank Nucleotide #: NM_172138.1
• UniProt Accession #: Q8IZJ0; Q45KQ8; Q6VN55; Q8IWL7
• Molecular Weight: 22,288 Da
• NCBI Official Full Name: interferon lambda-2
• NCBI Official Synonym Full Names: interferon lambda 2
• NCBI Official Symbol: IFNL2
• NCBI Official Synonym Symbols: IL28A; IL-28A
• NCBI Protein Information: interferon lambda-2
• UniProt Protein Name: Interferon lambda-2
• UniProt Gene Name: IFNL2
• UniProt Synonym Gene Names: IL28A; ZCYTO20; IFN-lambda-2; IL-28A

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN-stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type-selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. Plays a significant role in the antiviral immune defense in the intestinal epithelium. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression.