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Typical Testing Data/Standard Curve (for reference only)

Human IL-28A ELISA Kit | IFNL2 elisa kit

Human IL-28A ELISA Kit

Gene Names
IFNL2; IL28A; IL-28A
Reactivity
Human
Applications
ELISA
Synonyms
IL-28A; Human IL-28A ELISA Kit; IL28A; ZCYTO20; Interferon lambda-2; IFN-lambda-2; Cytokine Zcyto20; Interleukin-28A; IFNL2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
200
Applicable Applications for IFNL2 elisa kit
Sandwich ELISA (SE)
Samples
Cell Culture Supernatants, Serum, Plasma
Sensitivity
<30 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IFNL2 elisa kit
Principle of the Assay: MBS Human IL-28A ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28A in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human IL-28A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Interferon lambda-2, also called IFNL2 or IL28A is a class II cytokine receptor ligand related to type I interferons. This gene is mapped to 19q13.2. This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster. It up-regulates MHC class I antigen expression. It also displays antitumor activity. The ligand/receptor complex seems to signal through the Jak-STAT pathway. This gene seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. It seems play a significant role in the antiviral immune defense in the intestinal epithelium.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,288 Da
NCBI Official Full Name
interferon lambda-2
NCBI Official Synonym Full Names
interferon, lambda 2
NCBI Official Symbol
IFNL2
NCBI Official Synonym Symbols
IL28A; IL-28A
NCBI Protein Information
interferon lambda-2; IFN-lambda-2; interleukin-28A; cytokine Zcyto20; interferon lambda 2; interferon-lambda 2; interleukin 28A (interferon, lambda 2)
UniProt Protein Name
Interferon lambda-2
Protein Family
UniProt Gene Name
IFNL2
UniProt Synonym Gene Names
IL28A; ZCYTO20; IFN-lambda-2; IL-28A
UniProt Entry Name
IFNL2_HUMAN

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.13

Cellular Component: extracellular space

Molecular Function: cytokine activity

Biological Process: positive regulation of immune response; mucosal immune response; JAK-STAT cascade; defense response to virus

Research Articles on IFNL2

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Product Notes

The Human IFNL2 ifnl2 (Catalog #AAA824735) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA824735 ELISA Kit recognizes Human IFNL2. AAA Biotech's IL-28A can be used in a range of immunoassay formats including, but not limited to, Sandwich ELISA (SE). Researchers should empirically determine the suitability of the IFNL2 ifnl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-28A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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