IP-10 (CXCL10) Recombinant Protein | IP-10 recombinant protein

IP-10 (CXCL10)

Cat. Num. AAA517100

• Gene Names: CXCL10; C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
• Applications: Cell Assay
• Synonyms: IP-10 (CXCL10); CXCL10; C-X-C motif chemokine 10; 10kDa interferon gamma-induced protein; Gamma-IP10; Small-inducible cytokine B10; IP10; IP-10 recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from 0.2 um filtered PBS solution, pH7.0.

• Applicable Applications for IP-10 recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1mg/ml. This solution can then be diluted into other buffers.
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Activity

(Sample Cell Assay Data: Determined by its ability to chemoattract human T-Lymphocytes using a concentration of 10-50 ng/mL.)

SDS-Page

(Purity: Recombinant Human IP10 (CXCL10) resolved on a 17% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for IP-10 recombinant protein

Recombinant Human IP-10 (CXCL10) was expressed in E Coli cells. The protein accession number is P02778
IP-10 shows homology to PF-4 (platelet factor-4) and belongs to the family of chemotactic cytokines known as chemokines. The receptor for IP-10 is CXCR3. IP-10 has been shown to bind to the virus-encoded viroceptor M3. The expression of IP-10 from a variety of cells, including monocytes, endothelial cells, keratinocytes, and fibroblasts, is induced by IFN-gamma and TNF-alpha. Human neutrophils produce IP-10 in response to IFN-gamma in combination with either TNF-alpha or bacterial lipopolysaccharides. IP-10 probably also plays a role in regulation of the growth of immature hematopoietic progenitor cells. It has been shown to suppress in vitro colony formation of highly enriched cells expressing the cell surface marker CD34 in the presence of SCF, GM-CSF, or SCF and EPO, but not in their absence with the exception of SCF.

Product Categories/Family for IP-10 recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 149999382
• NCBI GeneID: 3627
• NCBI Accession #: NP_001556.2
• NCBI GenBank Nucleotide #: NM_001565.3
• UniProt Accession #: P02778; Q96QJ5
• Molecular Weight: 10,881 Da
• NCBI Official Full Name: C-X-C motif chemokine 10
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 10
• NCBI Official Symbol: CXCL10
• NCBI Official Synonym Symbols: C7; IFI10; INP10; IP-10; crg-2; mob-1; SCYB10; gIP-10
• NCBI Protein Information: C-X-C motif chemokine 10; gamma IP10; gamma-IP10; small-inducible cytokine B10; interferon-inducible cytokine IP-10; 10 kDa interferon gamma-induced protein; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X
• UniProt Protein Name: C-X-C motif chemokine 10
• UniProt Gene Name: CXCL10
• UniProt Synonym Gene Names: INP10; SCYB10; Gamma-IP10; IP-10
• UniProt Entry Name: CXL10_HUMAN

NCBI Description

This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects, including stimulation of monocytes, natural killer and T-cell migration, and modulation of adhesion molecule expression. [provided by RefSeq, Jul 2008]

Uniprot Description

CXCL10: Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Motility/polarity/chemotaxis; Secreted; Chemokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: heparin binding; protein binding; CXCR3 chemokine receptor binding; chemokine activity; cAMP-dependent protein kinase regulator activity; receptor binding

Biological Process: muscle development; blood circulation; protein secretion; positive regulation of leukocyte chemotaxis; positive regulation of cAMP metabolic process; chemotaxis; signal transduction; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; cell surface receptor linked signal transduction; response to vitamin D; cell-cell signaling; positive regulation of cell proliferation; response to gamma radiation; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of release of sequestered calcium ion into cytosol; response to cold; inflammatory response; negative regulation of myoblast differentiation; regulation of protein kinase activity; defense response to virus