Human Fibulin 1 (FBLN1) RTU ELISA Kit | FBLN1 rtu elisa kit

Human Fibulin 1 (FBLN1) ELISA Kit

Cat. Num. AAA459217

• Gene Names: FBLN1; FBLN; FIBL1
• Reactivity: Human
• Synonyms: Fibulin 1 (FBLN1); Human Fibulin 1 (FBLN1) ELISA Kit; FBLN1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 683

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.468-30ng/mL
• Sensitivity: 0.15ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FBLN1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBLN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FBLN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FBLN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FBLN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 34734062
• NCBI GeneID: 2192
• NCBI Accession #: NP_001987.2
• NCBI GenBank Nucleotide #: NM_001996.3
• UniProt Accession #: P23142; P23143; P23144; P37888; Q5TIC4; Q8TBH8; Q9HBQ5; Q9UC21; Q9UGR4; Q9UH41; B0QY42; B1AHL4
• Molecular Weight: 74,434 Da
• NCBI Official Full Name: fibulin-1 isoform C
• NCBI Official Synonym Full Names: fibulin 1
• NCBI Official Symbol: FBLN1
• NCBI Official Synonym Symbols: FBLN; FIBL1
• NCBI Protein Information: fibulin-1
• UniProt Protein Name: Fibulin-1
• UniProt Gene Name: FBLN1
• UniProt Synonym Gene Names: FIBL-1

NCBI Description

Fibulin 1 is a secreted glycoprotein that becomes incorporated into a fibrillar extracellular matrix. Calcium-binding is apparently required to mediate its binding to laminin and nidogen. It mediates platelet adhesion via binding fibrinogen. Four splice variants which differ in the 3' end have been identified. Each variant encodes a different isoform, but no functional distinctions have been identified among the four variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Incorporated into fibronectin-containing matrix fibers. May play a role in cell adhesion and migration along protein fibers within the extracellular matrix (ECM). Could be important for certain developmental processes and contribute to the supramolecular organization of ECM architecture, in particular to those of basement membranes. Has been implicated in a role in cellular transformation and tumor invasion, it appears to be a tumor suppressor. May play a role in haemostasis and thrombosis owing to its ability to bind fibrinogen and incorporate into clots. Could play a significant role in modulating the neurotrophic activities of APP, particularly soluble APP.